Carney Bonnie C, Ortiz Rachel T, Bullock Rachael M, Prindeze Nicholas J, Moffatt Lauren T, Robson Martin C, Shupp Jeffrey W
Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.
Department of Surgery, University of South Florida, Tampa.
Eplasty. 2014 Dec 10;14:e45. eCollection 2014.
Drawtex's ability to remove pathogens and associated virulence factors has been demonstrated in vitro. A model of burn wound infection was used to characterize the in vivo impact of this dressing on infection and wound healing.
Paired burn wounds were created on the dorsum of Sprague Dawley rats and were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Animals were divided into 2 groups, half with wounds that received experimental dressing and the remaining half with control dressing-treated wounds. Dressings remained in place through 3, 6, 9, or 14 days after injury, and methicillin-resistant S aureus and virulence factors were quantified. Laser Doppler imaging was used to examine wound perfusion, and local host immune response was assessed through the quantification of mRNA expression.
By day 3, less methicillin-resistant S aureus was measured in wounds treated with experimental-dressing compared to control-dressing wounds. Quantities remained lower in the experimental group through day 14 (P < .001). More methicillin-resistant S aureus was quantified in the experimental dressing itself than in control dressing at all time points (P < .05). Experimental dressing-treated wounds contained less toxic shock syndrome toxin 1 and Panton-Valentine leukocidin than controls (P < .01) on days 6, 9, and 14. Induction of toll-like receptor 2, NOD-like receptor family, pyrin domain containing 3, and interleukin 6 was significantly lower in experimental-dressing treated wounds than in controls on days 6 and 9 (P < .05).
The hydroconductive dressing provided a significant reduction in pathogen and virulence factors compared to a control dressing. As a result of clearance of virulence factors from the wound bed, a requisite alteration in host innate immune response was observed.
Drawtex去除病原体及相关毒力因子的能力已在体外得到证实。采用烧伤创面感染模型来表征这种敷料对感染和创面愈合的体内影响。
在Sprague Dawley大鼠背部制造成对的烧伤创面，并接种耐甲氧西林金黄色葡萄球菌（MRSA）。动物分为2组，一半创面接受实验敷料，另一半接受对照敷料处理。敷料在伤后3、6、9或14天内一直保留，对耐甲氧西林金黄色葡萄球菌和毒力因子进行定量。使用激光多普勒成像检查创面灌注，并通过定量mRNA表达评估局部宿主免疫反应。
到第3天，与对照敷料处理的创面相比，实验敷料处理的创面中检测到的耐甲氧西林金黄色葡萄球菌较少。在第14天之前，实验组的数量一直较低（P <.001）。在所有时间点，实验敷料本身中耐甲氧西林金黄色葡萄球菌的定量均高于对照敷料（P <.05）。在第6、9和14天，实验敷料处理的创面中含有的毒性休克综合征毒素1和杀白细胞素比对照组少（P <.01）。在第6天和第9天，实验敷料处理的创面中Toll样受体2、含核苷酸结合寡聚化结构域样受体家族、含pyrin结构域的3和白细胞介素6的诱导明显低于对照组（P <.05）。
与对照敷料相比，这种导水敷料能显著减少病原体和毒力因子。由于从创面床清除了毒力因子，观察到宿主固有免疫反应发生了必要的改变。
