State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, P.R. China.
ACS Appl Mater Interfaces. 2015 Jan 14;7(1):935-40. doi: 10.1021/am5075216. Epub 2015 Jan 2.
We propose a novel method to obtain versatile droplets arrays on a regional hydrophilic chip that is fabricated by PDMS soft lithography and regional plasma treatment. It enables rapid liquid dispensation and droplets array formation just making the chip surface in contact with solution. By combining this chip with a special Christmas Tree structure, the droplets array with concentrations in gradient is generated. It possesses the greatly improved performance of convenience and versatility in bioscreening and biosensing. For example, high throughput condition screening of toxic tests of CdSe quantum dots on HL-60 cells are conducted and cell death rates are successfully counted quickly and efficiently. Furthermore, a rapid biosensing approach for cancer biomarkers carcinoma embryonic antigen (CEA) is developed via magnetic beads (MBs)-based sandwich immunoassay methods.
我们提出了一种新颖的方法，通过 PDMS 软光刻和局部等离子体处理来在局部亲水芯片上获得多功能的液滴阵列。只需使芯片表面与溶液接触，就可以实现快速的液体分配和液滴阵列的形成。通过将这种芯片与特殊的圣诞树结构相结合，可以生成具有浓度梯度的液滴阵列。它在生物筛选和生物传感方面具有极大地提高了便利性和通用性的性能。例如，可以在 HL-60 细胞上进行 CdSe 量子点毒性测试的高通量条件筛选，并且可以快速有效地成功计数细胞死亡率。此外，还通过基于磁性珠（MB）的三明治免疫测定方法开发了用于癌症生物标志物癌胚抗原（CEA）的快速生物传感方法。
