Papagiannopoulos Aristeidis, Christoulaki Anastasia, Spiliopoulos Nikolaos, Vradis Alexandros, Toprakcioglu Chris, Pispas Stergios
Theoretical and Physical Chemistry Institute , National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece.
Langmuir. 2015 Jan 20;31(2):685-94. doi: 10.1021/la504873h. Epub 2015 Jan 6.
We present a study of the interaction of the positively charged model protein lysozyme with the negatively charged amphiphilic diblock polyelectrolyte micelles of poly(tert-butylstyrene-b-sodium (sulfamate/carboxylate)isoprene) (PtBS-b-SCPI) on the silver/water interface. The adsorption kinetics are monitored by surface plasmon resonance, and the surface morphology is probed by atomic force microscopy. The micellar adsorption is described by stretched-exponential kinetics, and the micellar layer morphology shows that the micelles do not lose their integrity upon adsorption. The complexation of lysozyme with the adsorbed micellar layers depends on the micelles arrangement and density in the underlying layer, and lysozyme follows the local morphology of the underlying roughness. When the micellar adsorbed amount is small, the layers show low capacity in protein complexation and low resistance in loading. When the micellar adsorbed amount is high, the situation is reversed. The adsorbed layers both with or without added protein are found to be irreversibly adsorbed on the Ag surface.
我们展示了一项关于带正电荷的模型蛋白溶菌酶与聚（叔丁基苯乙烯 - b - （氨基磺酸盐/羧酸盐）异戊二烯钠）（PtBS - b - SCPI）带负电荷的两亲性双嵌段聚电解质胶束在银/水界面相互作用的研究。通过表面等离子体共振监测吸附动力学，并用原子力显微镜探测表面形态。胶束吸附由拉伸指数动力学描述，胶束层形态表明胶束在吸附时不会失去其完整性。溶菌酶与吸附的胶束层的络合取决于下层中胶束的排列和密度，并且溶菌酶遵循下层粗糙度的局部形态。当胶束吸附量较小时，这些层在蛋白质络合方面表现出低容量且在负载方面具有低阻力。当胶束吸附量较高时，情况则相反。发现添加或未添加蛋白质的吸附层都不可逆地吸附在银表面。
