木薯（Manihot esculenta Krantz）基因组含有在块根发育过程中差异表达的KNOX基因。

Cassava (Manihot esculenta Krantz) genome harbors KNOX genes differentially expressed during storage root development.

作者信息

Guo D, Li H L, Tang X, Peng S Q

机构信息

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

出版信息

Genet Mol Res. 2014 Dec 18;13(4):10714-26. doi: 10.4238/2014.December.18.13.

DOI:10.4238/2014.December.18.13

PMID:25526192

Abstract

In plants, homeodomain proteins play a critical role in regulating various aspects of plant growth and development. KNOX proteins are members of the homeodomain protein family. The KNOX transcription factors have been reported from Arabidopsis, rice, and other higher plants. The recent publication of the draft genome sequence of cassava (Manihot esculenta Krantz) has allowed a genome-wide search for M. esculenta KNOX (MeKNOX) transcription factors and the comparison of these positively identified proteins with their homologs in model plants. In the present study, we identified 12 MeKNOX genes in the cassava genome and grouped them into two distinct subfamilies based on their domain composition and phylogenetic analysis. Furthermore, semi-quantitative reverse transcription polymerase chain reaction analysis was performed to elucidate the expression profiles of these genes in different tissues and during various stages of root development. The analysis of MeKNOX expression profiles of indicated that 12 MeKNOX genes display differential expressions either in their transcript abundance or expression patterns.

摘要

在植物中，同源结构域蛋白在调节植物生长发育的各个方面发挥着关键作用。KNOX蛋白是同源结构域蛋白家族的成员。拟南芥、水稻和其他高等植物中均已报道了KNOX转录因子。木薯（Manihot esculenta Krantz）基因组序列草图的最新公布，使得能够在全基因组范围内搜索木薯KNOX（MeKNOX）转录因子，并将这些已明确鉴定的蛋白与其在模式植物中的同源物进行比较。在本研究中，我们在木薯基因组中鉴定出12个MeKNOX基因，并根据其结构域组成和系统发育分析将它们分为两个不同的亚家族。此外，进行了半定量逆转录聚合酶链反应分析，以阐明这些基因在不同组织以及根发育的各个阶段的表达谱。MeKNOX表达谱分析表明，12个MeKNOX基因在转录丰度或表达模式上均表现出差异表达。