大戟科植物中法尼基二磷酸合酶基因的结构保守性与差异表达
Structure Conservation and Differential Expression of Farnesyl Diphosphate Synthase Genes in Euphorbiaceous Plants.
作者信息
Guo Dong, Li Hui-Liang, Peng Shi-Qing
机构信息
Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
出版信息
Int J Mol Sci. 2015 Sep 15;16(9):22402-14. doi: 10.3390/ijms160922402.
Farnesyl diphosphate synthase (FPS) is a key enzyme of isoprenoids biosynthesis. However, knowledge of the FPSs of euphorbiaceous species is limited. In this study, ten FPSs were identified in four euphorbiaceous plants. These FPSs exhibited similar exon/intron structure. The deduced FPS proteins showed close identities and exhibited the typical structure of plant FPS. The members of the FPS family exhibit tissue expression patterns that vary among several euphorbiaceous plant species under normal growth conditions. The expression profiles reveal spatial and temporal variations in the expression of FPSs of different tissues from Euphorbiaceous plants. Our results revealed wide conservation of FPSs and diverse expression in euphorbiaceous plants during growth and development.
法尼基二磷酸合酶（FPS）是类异戊二烯生物合成的关键酶。然而，关于大戟科植物中FPS的了解有限。在本研究中，在四种大戟科植物中鉴定出了10个FPS。这些FPS表现出相似的外显子/内含子结构。推导的FPS蛋白显示出密切的同源性，并具有植物FPS的典型结构。FPS家族成员在正常生长条件下，在几种大戟科植物物种中表现出不同的组织表达模式。表达谱揭示了大戟科植物不同组织中FPS表达的时空变化。我们的结果表明，FPS在大戟科植物生长发育过程中具有广泛的保守性和多样的表达。
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