Zhang Lijuan, He Nan, Lu Chao
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
Anal Chem. 2015 Jan 20;87(2):1351-7. doi: 10.1021/ac5041605. Epub 2015 Jan 6.
The emergence of aggregation-induced emission (AIE) has opened up a new avenue for scientists. There is a great demand for the development of a new generation chemiluminescence resonance energy transfer (CRET) acceptors with AIE characteristics due to the aggregation-caused chemiluminescence (CL) quenching effect commonly observed in the conventional fluorophore CL acceptors at high concentrations. However, the systematical studies involving in AIE-amplified CL are still scarce. Herein, it is the first report that the gold nanocluster aggregates (a type of well-defined AIE molecules) are used to study their influence on the bis(2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 CL reaction. Interestingly, the AIE molecules in the diluted solution are unable to boost the CL signal of the TCPO-H2O2 system, but their aggregates display a strongly enhanced CL emission compared to their counterparts of fluorophore molecules, thanks to the unique AIE effect of gold nanoclusters. In comparison to rhodamine B with the aid of an imidazole catalyst, the detection limit of the gold nanocluster aggregate-amplified CL probe for H2O2 (S/N = 3) is low in the absence of any catalyst. Finally, the other two typical AIE molecules, Au(I)-thiolate complexes and 9,10-bis[4-(3-sulfonatopropoxyl)-styryl]anthracene (BSPSA), are investigated to verify the generality of the AIE molecule-amplified CL emissions. These results demonstrate effective access to highly fluorescent AIE molecules with practical applications in avoiding the aggregation-induced CL quenching at high concentrations, which can be expected to provide a novel and sensitive platform for the CL amplified detection.
聚集诱导发光（AIE）的出现为科学家们开辟了一条新途径。由于在传统荧光团化学发光（CL）受体中高浓度时普遍观察到聚集导致的化学发光猝灭效应，因此迫切需要开发具有AIE特性的新一代化学发光共振能量转移（CRET）受体。然而，涉及AIE增强化学发光的系统研究仍然很少。在此，首次报道了金纳米团簇聚集体（一种定义明确的AIE分子）用于研究它们对双（2,4,6-三氯苯基）草酸酯（TCPO）-H₂O₂化学发光反应的影响。有趣的是，稀释溶液中的AIE分子无法增强TCPO-H₂O₂体系的化学发光信号，但与荧光团分子的对应物相比，它们的聚集体显示出强烈增强的化学发光发射，这得益于金纳米团簇独特的AIE效应。与借助咪唑催化剂的罗丹明B相比，在没有任何催化剂的情况下，金纳米团簇聚集体增强化学发光探针检测H₂O₂的检测限（S/N = 3）较低。最后，研究了另外两种典型的AIE分子，Au（I）-硫醇盐配合物和9,10-双[4-（3-磺丙氧基）-苯乙烯基]蒽（BSPSA），以验证AIE分子增强化学发光发射的普遍性。这些结果表明，可以有效地获得具有实际应用价值的高荧光AIE分子，避免高浓度下的聚集诱导化学发光猝灭，有望为化学发光放大检测提供一个新颖且灵敏的平台。
