Espregueira Themudo Gonçalo, Rufino Ana C, Campos Paula F
CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 177, 4050-123 Porto, Portugal.
Museu da Ciência, Universidade de Coimbra, Laboratorio Chimico, Largo Marquês de Pombal, 3000-272 Coimbra, Portugal.
Mol Phylogenet Evol. 2015 Feb;83:242-9. doi: 10.1016/j.ympev.2014.12.001. Epub 2014 Dec 17.
The giant sable antelope is one of the most endangered African bovids. Populations of this iconic animal, the national symbol of Angola, were recently rediscovered, after many decades of presumed extinction. Even so, their numbers are scarce and hence conservation plans are essential. However, fundamental information such as its taxonomic position, time of divergence and degree of genetic variation are still lacking. Here, we used a museum preserved horn as a source of DNA to describe, for the first time, the complete mitochondrial genome of the giant sable antelope, and provide insights into its evolutionary history. Reads generated by shotgun sequencing were mapped against the mitochondrial genome of common sable antelope and the nuclear genomes of cow and sheep. Phylogenetic reconstruction and divergence time estimate give support to the monophyly of the giant sable and a maximum divergence time of 170 thousand years to the closest subspecies. About 7% of the nuclear genome was mapped against the reference. The genetic resources reported here are now available for future work in the field of conservation genetics and phylogeny, in this and related species.
巨型貂羚是非洲最濒危的牛科动物之一。这种标志性动物是安哥拉的国家象征，在被认为已灭绝数十年后，其种群最近被重新发现。即便如此，它们的数量仍然稀少，因此保护计划至关重要。然而，关于其分类地位、分化时间和遗传变异程度等基本信息仍然缺乏。在此，我们使用博物馆保存的角作为DNA来源，首次描述了巨型貂羚的完整线粒体基因组，并深入了解其进化历史。通过鸟枪法测序产生的 reads 与普通貂羚的线粒体基因组以及牛和羊的核基因组进行比对。系统发育重建和分化时间估计支持巨型貂羚的单系性以及与最接近亚种的最大分化时间为17万年。约7%的核基因组与参考基因组进行了比对。本文报道的遗传资源现在可用于该物种及相关物种在保护遗传学和系统发育领域的未来研究工作。
