二氯二氨合铂（II），[PtCl2(NH3)2]，异构体与锌指的反应。

The reaction of dichlorodiammineplatinum(II), [PtCl2(NH3)2], isomers with zinc fingers.

作者信息

Tsotsoros Samantha D, Qu Yun, Farrell Nicholas P

机构信息

Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA.

出版信息

J Inorg Biochem. 2015 Feb;143:117-22. doi: 10.1016/j.jinorgbio.2014.12.003. Epub 2014 Dec 10.

DOI:10.1016/j.jinorgbio.2014.12.003

PMID:25528679

Abstract

The interaction of cis-DDP and trans-DDP (DDP=[PtCl2(NH3)2]) with the C-terminal zinc finger (ZF2) of the HIVNCp7 nucleocapsid protein was investigated by fluorescence, circular dichroism, mass spectrometry, and {(1)H, (15)N} HSQC (heteronuclear single quantum coherence) NMR spectroscopy. The rate of reaction differed significantly for the two compounds, with the trans isomer reacting in a significantly faster manner, as expected. {(1)H, (15)N} HSQC NMR of (15)N-labeled compounds with the ZF2 showed the appearance of several new (15)N peaks, consistent with sulfur binding and formation of Pt-S species. Mass spectrometry confirmed the formation of several different Pt-apopeptide/ZF2 adducts. Circular dichroism and fluorescence spectroscopy also indicated conformational changes upon binding while a 33% decrease in fluorescence of the unique tryptophan residue was seen in 72h upon complexation of the cis isomer, while the trans isomer quenched 50% in just 24h.

摘要

通过荧光、圆二色性、质谱以及{¹H, ¹⁵N} HSQC（异核单量子相干）核磁共振光谱法，研究了顺铂和反铂（DDP = [PtCl₂(NH₃)₂]）与HIV NCp7核衣壳蛋白C端锌指（ZF2）的相互作用。两种化合物的反应速率差异显著，正如预期的那样，反式异构体的反应速度明显更快。¹⁵N标记的化合物与ZF2的{¹H, ¹⁵N} HSQC核磁共振显示出现了几个新的¹⁵N峰，这与硫结合以及Pt-S物种的形成一致。质谱证实形成了几种不同的Pt-脱辅基肽/ZF2加合物。圆二色性和荧光光谱也表明结合后会发生构象变化，顺式异构体络合72小时后，唯一色氨酸残基的荧光降低了33%，而反式异构体在仅24小时内就淬灭了50%。