Bakir Nur, Bozkuş Hasan Mert, Koldemir Gündüz Meliha, Çağatay Penbe, Taşkın Mustafa, Süsleyici Duman Belgin
Marmara University, Faculty of Science and Arts, Department of Biology, İstanbul, Turkey.
Istanbul University, Istanbul Medical Faculty, Department of Biostatistic and Medical Informatics, İstanbul, Turkey.
Acta Med Acad. 2014;43(2):144-54. doi: 10.5644/ama2006-124.113.
This study examined the association of C-58T genotypes with obesity/hypertension related parameters and serum lipids in obese (n=108) and non-obese (n=80) patients.
Bradykinin receptor (B₂R) C-58T genotypes were determined by PCR-RFLP.
B₂R gene C-58T frequencies for T/T (homozygous wild type), T/C (heterozygous) and C/C (homozygous polymorphic) genotypes for obese and non-obese patients were respectively: 36.1%, 37.5%; 45.4%, 52.5% and 18.5%, 10%. Obese patients using diuretic medication had lower C/C genotype frequency compared to T/T and T/C genotypes. Total cholesterol (T-Chol) (p=0.035) levels were found to be associated with B₂R C-58T polymorphism, where the T/T genotype had higher total cholesterol levels compared to the T/C genotype in obese patients. Non-obese patients using oral antidiabetic medication had higher C/C genotype frequency than that of T/T and T/C genotypes. Waist circumference (p=0.016) and diastolic blood pressure (p=0.01) levels were elevated in the non-obese subjects with the C/C genotype compared to T/C and T/T.
Although B₂R C-58T gene polymorphism was not found to be effective on obesity with logistic regression analysis in the whole study population in obese subjects, the T-Chol decreasing effect of the B₂R gene C allele and the higher waist circumference measurements in the non-obese subjects may indicate there may be a link between B₂R gene C-58T polymorphism and obesity in study populations of higher numbers.
本研究调查了肥胖（n = 108）和非肥胖（n = 80）患者中C-58T基因型与肥胖/高血压相关参数及血脂之间的关联。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法测定缓激肽受体（B₂R）C-58T基因型。
肥胖和非肥胖患者中，B₂R基因C-58T基因型中T/T（纯合野生型）、T/C（杂合子）和C/C（纯合多态性）基因型的频率分别为：36.1%，37.5%；45.4%，52.5%和18.5%，10%。使用利尿剂的肥胖患者中，C/C基因型频率低于T/T和T/C基因型。发现总胆固醇（T-Chol）（p = 0.035）水平与B₂R C-58T多态性相关，在肥胖患者中，T/T基因型的总胆固醇水平高于T/C基因型。使用口服抗糖尿病药物的非肥胖患者中，C/C基因型频率高于T/T和T/C基因型。与T/C和T/T基因型相比，C/C基因型的非肥胖受试者的腰围（p = 0.016）和舒张压（p = 0.01）水平升高。
尽管在整个研究人群中，通过逻辑回归分析未发现B₂R C-58T基因多态性对肥胖有影响，但在肥胖受试者中，B₂R基因C等位基因具有降低T-Chol的作用，而在非肥胖受试者中腰围测量值较高，这可能表明在更多数量的研究人群中，B₂R基因C-58T多态性与肥胖之间可能存在联系。
