Takimoto T, Tanaka S, Ishikawa S, Umeda R
Department of Otolaryngology, School of Medicine, Kanazawa University, Japan.
Auris Nasus Larynx. 1989;16(2):109-15. doi: 10.1016/s0385-8146(89)80042-2.
Nineteen human nasopharyngeal tissue specimens biopsied from patients with suspected nasopharyngeal carcinoma (NPC) and 15 adenoid tissue specimens obtained by surgery were successfully grown in culture without bacterial and fungal contamination. Fibroblastic growth developed in all of the cultures. In some cases, epithelial growth was also seen among the fibroblastic growth. The cells became round and floated in the culture fluid between 19th and 57th day of culture in 6 out of 10 NPC tissue cultures, 3 out of 9 tissue cultures of apparently normal nasopharyngeal mucosa of patients referred for exclusion of NPC, and 4 out of 15 adenoid tissue cultures. The floating cells consisted of B-lymphoblastoid cells and were positive Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA). The data suggest that a small number of B-lymphocytes in the human nasopharynx serve as a reservoir for EBV.
从疑似鼻咽癌（NPC）患者身上活检获取的19份人类鼻咽组织标本，以及通过手术获得的15份腺样体组织标本，在无细菌和真菌污染的情况下成功培养生长。所有培养物中均出现成纤维细胞生长。在某些情况下，成纤维细胞生长中也可见上皮生长。在10份NPC组织培养物中的6份、因排除NPC而转诊的患者的9份明显正常鼻咽黏膜组织培养物中的3份以及15份腺样体组织培养物中的4份中，细胞在培养的第19天至第57天之间变圆并漂浮在培养液中。漂浮细胞由B淋巴母细胞组成，且爱泼斯坦-巴尔病毒（EBV）相关核抗原（EBNA）呈阳性。数据表明，人类鼻咽中的少量B淋巴细胞是EBV的储存库。
