多胺和新霉素通过与相关的多磷酸肌醇相互作用来抑制纯化的质膜Ca2+泵。
Polyamines and neomycin inhibit the purified plasma-membrane Ca2+ pump by interacting with associated polyphosphoinositides.
作者信息
Missiaen L, Wuytack F, Raeymaekers L, De Smedt H, Casteels R
机构信息
Physiological Laboratory, K. U. Leuven, Campus Gasthuisberg, Belgium.
出版信息
Biochem J. 1989 Aug 1;261(3):1055-8. doi: 10.1042/bj2611055.
Abstract
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin. Millimolar concentrations of spermine and to a lesser extent of spermidine decreased the ATPase activity in the presence of phosphatidylinositol 4,5-bisphosphate (PIP2), without affecting the stimulation by phosphatidylinositol 4-phosphate (PIP). Sub-millimolar concentrations of neomycin inhibited the stimulation of the ATPase by PIP and by PIP2. Neomycin was more effective at the higher concentrations of PIP and PIP2. We discuss that these findings are compatible with the hypothesis that PIP and PIP2 bind to the ATPase and that several of these molecules have to be available to stimulate the ATPase.
摘要
我们研究了精胺、亚精胺、腐胺和新霉素对质膜Ca2+泵活性及其受带负电荷磷脂和钙调蛋白刺激的影响。毫摩尔浓度的精胺以及浓度稍低的亚精胺在磷脂酰肌醇4,5-二磷酸（PIP2）存在的情况下降低了ATP酶活性，而不影响磷脂酰肌醇4-磷酸（PIP）的刺激作用。亚毫摩尔浓度的新霉素抑制了PIP和PIP2对ATP酶的刺激。新霉素在较高浓度的PIP和PIP2时更有效。我们讨论了这些发现与以下假设相符，即PIP和PIP2与ATP酶结合，并且这些分子中的几个必须存在才能刺激ATP酶。
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本文引用的文献
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Neomycin inhibits inositol phosphate formation in human platelets stimulated by thrombin but not other agonists.新霉素可抑制凝血酶刺激的人血小板中肌醇磷酸的形成，但对其他激动剂无此作用。
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