钒酸盐对大鼠肝细胞中蛋白激酶的影响。
Effects of vanadate on protein kinases in rat hepatocytes.
作者信息
Villar-Palasi C, Guinovart J J, Gómez-Foix A M, Rodriguez-Gil J E, Bosch F
机构信息
Department of Biochemistry, Autonomous University of Barcelona, School of Veterinary Medicine, Spain.
出版信息
Biochem J. 1989 Sep 1;262(2):563-7. doi: 10.1042/bj2620563.
In rat hepatocytes, vanadate modifies neither the intracellular concentration of cyclic AMP nor the --cyclic AMP/+cyclic AMP activity ratio for cyclic AMP-dependent protein kinase. Vanadate can, however, counteract the increase in cyclic AMP and the increase in the --cyclic AMP/+cyclic AMP activity ratio of cyclic AMP-dependent protein kinase induced by glucagon. On the other hand, vanadate treatment of hepatocytes can produce a time- and concentration-dependent increase in cyclic AMP- and Ca2+-independent casein kinase activity. Maximal activation at the optimal time with 5 mM-vanadate was about 70% over control. A clear relationship was observed between the activation of casein kinase and the inactivation of glycogen synthase after vanadate treatment. These results suggest that casein kinase activity may be involved in vanadate actions in rat hepatocytes.
在大鼠肝细胞中，钒酸盐既不改变细胞内环磷酸腺苷（cAMP）的浓度，也不改变环磷酸腺苷依赖性蛋白激酶的 -cAMP/+cAMP活性比值。然而，钒酸盐可以抵消胰高血糖素诱导的环磷酸腺苷增加以及环磷酸腺苷依赖性蛋白激酶的 -cAMP/+cAMP活性比值的增加。另一方面，用钒酸盐处理肝细胞可使环磷酸腺苷和钙离子非依赖性酪蛋白激酶活性随时间和浓度依赖性增加。在最佳时间用5 mM钒酸盐处理时，最大激活程度比对照高约70%。钒酸盐处理后，观察到酪蛋白激酶的激活与糖原合酶的失活之间存在明显关系。这些结果表明酪蛋白激酶活性可能参与了钒酸盐在大鼠肝细胞中的作用。
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