心钠素诱导的环磷酸鸟苷生成的竞争性肽拮抗剂。

Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production.

作者信息

Abell T J, Richards A M, Yandle T G, Espiner E A, Frampton C, Ikram H

机构信息

Department of Medicine, Princess Margaret Hospital, Christchurch, New Zealand.

出版信息

Biochem Biophys Res Commun. 1989 Oct 16;164(1):108-13. doi: 10.1016/0006-291x(89)91689-6.

DOI:10.1016/0006-291x(89)91689-6

PMID:2553010

Abstract

Atrial natriuretic factor (isoleucine ANF 101-126), cleaved ANF (isoleucine ANF 101-105/106-126) and des (Gln 18, Ser 19, Gly 20, Leu 21, Gly 22) ANF 4-23-NH2 (C-ANF 4-23) stimulated cyclic guanosine monophosphate production (cGMP) by rat aortic vascular smooth muscle cells (VSMC) in culture. Cleaved ANF and ANF C4-23 also antagonised or diminished the response to ANF 101-126. Agonist and antagonist actions of both peptides were dose-related. In contrast, prepro ANF (104-123), an ANF precursor fragment, exhibited no agonist or antagonist effect on cGMP production.

摘要

心房利钠因子（异亮氨酸心房利钠因子101 - 126）、裂解型心房利钠因子（异亮氨酸心房利钠因子101 - 105/106 - 126）和去（谷氨酰胺18、丝氨酸19、甘氨酸20、亮氨酸21、甘氨酸22）心房利钠因子4 - 23 - NH2（C型心房利钠因子4 - 23）可刺激培养的大鼠主动脉血管平滑肌细胞（VSMC）产生环磷酸鸟苷（cGMP）。裂解型心房利钠因子和心房利钠因子C4 - 23也可拮抗或减弱对心房利钠因子101 - 126的反应。两种肽的激动剂和拮抗剂作用均与剂量相关。相比之下，前体心房利钠因子（104 - 123），一种心房利钠因子前体片段，对cGMP的产生没有激动剂或拮抗剂作用。