Yumura-Yagi K, Hara J, Terada N, Ishihara S, Tawa A, Takihara Y, Champagne E, Minden M D, Mak T W, Kawa-Ha K
Department of Pediatrics, Osaka University Hospital, Osaka, Japan.
Blood. 1989 Nov 1;74(6):2103-11.
We analyzed the rearrangement and expression of T-cell receptor (TCR) genes, including the recently identified TCR delta gene, in 21 patients with T-lineage leukemia/lymphoma. Among 8 patients with CD3-, CD4-, and CD8- (group I), 2 patients showed germline configuration of the TCR delta, gamma, beta, and alpha genes and 1 patient demonstrated only TCR delta gene rearrangement. All nine patients with CD3-, CD4+, and/or CD8+ (group II) showed concomitant rearrangements of the TCR delta, gamma, and beta genes. TCR alpha gene rearrangement was also observed in two patients. Three of four patients with CD3+ (group III) showed rearrangement of the TCR alpha gene with deletion of both alleles or of a single allele of the TCR delta gene. With Northern blot analysis, full-length transcripts of the TCR delta gene were detected in 3 of 15 examined patients. All were restricted to group I or group II. In contrast, full-length transcripts of TCR beta and alpha were observed mainly in samples from groups II and III. Based on these findings, rearrangement of the TCR delta gene may be the earliest event in T-cell differentiation, preceding rearrangements of the other TCR genes.
我们分析了21例T细胞系白血病/淋巴瘤患者中T细胞受体（TCR）基因的重排和表达情况，其中包括最近发现的TCRδ基因。在8例CD3 -、CD4 -和CD8 -（第一组）患者中，2例患者的TCRδ、γ、β和α基因呈种系构型，1例患者仅表现出TCRδ基因重排。所有9例CD3 -、CD4 +和/或CD8 +（第二组）患者均表现出TCRδ、γ和β基因的同时重排。在2例患者中还观察到TCRα基因重排。4例CD3 +（第三组）患者中有3例表现出TCRα基因重排，同时TCRδ基因的两个等位基因或单个等位基因缺失。通过Northern印迹分析，在15例受检患者中的3例检测到TCRδ基因的全长转录本。所有这些患者均局限于第一组或第二组。相比之下，TCRβ和α的全长转录本主要在第二组和第三组的样本中观察到。基于这些发现，TCRδ基因重排可能是T细胞分化中最早发生的事件，早于其他TCR基因的重排。
