Irshad Muhammad, Asgher Muhammad, Anwar Zahid, Ahmad Aftab
Nat Prod Commun. 2014 Nov;9(11):1649-54.
In the last several years, in serious consideration of the worldwide economic and environmental issues there has been an increasing research interest in the value of naturally occurring bio-sourced materials. Agro-industrial based biomass comprised of pectin is an inexpensive, renewable, abundant natural resource that could be utilized for large-scale and cost-effective production of natural products i.e., pectinolytics. Pectinolytics are one of the most widely distributed enzymes in bacteria, fungi and plants. From ancient times to date, many methods have been introduced to improve the optimization of pectinolytics to obtain high yields of maximal purity. To expand the range of natural bio-resources the rapidly evolving tools of biotechnology can lower the conversion costs and also enhance target yield of the product of interest. This green biotechnology presents a promising approach to convert most of the agricultural materials into a value-added product with multiple applications. Major advances have already been achieved in recent years in order to obtain high levels of purity with optimal yields. The present review begins with an overview of pectinolytics and their physico-chemical features, and their specific role with classification based on pectic materials. Information is also given on the culture influences and potential sources of pectinolytics, followed by a brief summary of various industrial and biotechnological applications and future considerations.
在过去几年中，鉴于全球经济和环境问题，人们对天然生物源材料的价值研究兴趣日益浓厚。以农业产业为基础、由果胶构成的生物质是一种廉价、可再生且丰富的自然资源，可用于大规模且经济高效地生产天然产物，即果胶分解酶。果胶分解酶是细菌、真菌和植物中分布最广泛的酶之一。从古至今，人们引入了许多方法来优化果胶分解酶以获得高产量和最大纯度。为了扩大天然生物资源的范围，快速发展的生物技术工具可以降低转化成本，并提高目标产物的产量。这种绿色生物技术为将大多数农业材料转化为具有多种用途的增值产品提供了一种有前景的方法。近年来，为了以最佳产量获得高纯度，已经取得了重大进展。本综述首先概述果胶分解酶及其物理化学特性，以及它们基于果胶材料分类的具体作用。还介绍了培养条件的影响和果胶分解酶的潜在来源，随后简要总结了各种工业和生物技术应用以及未来的考虑因素。
