不同方法对产KPC碳青霉烯酶肺炎克雷伯菌进行庆大霉素药敏试验的准确性
Accuracy of different methods for susceptibility testing of gentamicin with KPC carbapenemase-producing Klebsiella pneumoniae.
作者信息
Arena Fabio, Giani Tommaso, Vaggelli Guendalina, Terenzi Giovanni, Pecile Patrizia, Rossolini Gian Maria
机构信息
Department of Medical Biotechnologies, University of Siena, Siena, Italy.
Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.
出版信息
Diagn Microbiol Infect Dis. 2015 Feb;81(2):132-4. doi: 10.1016/j.diagmicrobio.2014.10.011. Epub 2014 Nov 4.
Performance of Vitek2, Etest, and TREK broth microdilution (BMD) panels was evaluated versus reference CLSI BMD for gentamicin susceptibility testing with 57 bloodstream isolates of KPC-producing Klebsiella pneumoniae. Compared with reference BMD, the Essential Agreement and Categorical Agreement for TREK panels, Vitek2, and Etest were 91.2%, 31.6%, and 61.4%, respectively, and 86%, 21%, and 52.6%, respectively. Four very major discrepancies occurred with Vitek2. In these 4 strains, gentamicin resistance was associated with the presence of an armA aminoglycoside resistance determinant.
采用Vitek2、Etest和TREK肉汤微量稀释（BMD）方法对57株产KPC的肺炎克雷伯菌血流分离株进行庆大霉素敏感性检测，并与参考CLSI BMD方法进行比较。与参考BMD方法相比，TREK平板、Vitek2和Etest的基本一致性和分类一致性分别为91.2%、31.6%和61.4%，以及86%、21%和52.6%。Vitek2出现了4个非常主要的差异。在这4株菌株中，庆大霉素耐药与armA氨基糖苷类耐药决定簇的存在有关。
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