基质金属蛋白酶和一氧化氮合酶在脑动脉瘤中的病理生理作用

[Pathophysiological roles of matrix metalloproteinases and nitric oxide synthase in cerebral aneurysm].

作者信息

Wu Rile, Xu Xiaofei, Liu Aihua, Li Wenhui, Peng Tangming, Qian Zenghui, Wu Jing, Kang Huibin, Wu Zhongxue

机构信息

Inner Mongolia People's Hospital, Hohhot 010017, China.

Affiliated Beijing Tiantan Hospital, Capital Medical University, Beijing Neurosurgical Institute, Beijing 100050, China. Email:

出版信息

Zhonghua Yi Xue Za Zhi. 2014 Sep 23;94(35):2754-6.

PMID:25533982

Abstract

OBJECTIVE

To explore the expressions of matrix metalloproteinases-2 (MMP-2), matrix metalloproteinases-9 (MMP-9) and inducible nitric oxide synthase (iNOS) in cerebral aneurysms, compare them with normal brain vessels tissue so as to gain a better understanding of the pathogenesis of cerebral aneurysms.

METHODS

Twelve samples of cerebral aneurysms were obtained during operations and 10 cortical arteries as controls during surgery for temporal lobe epilepsy from 2009 to 2012 at Inner Mongolia People's Hospital and Beijing Tiantan Hospital. The activities of MMP-2, MMP-9 and iNOS in specimens were detected with spectrophotometry and substrate gel zymography.

RESULTS

The MMP-2 and MMP-9 levels in cerebral aneurysm group were (199 598 ± 125 288) gray scale area × mg⁻¹ × L⁻¹ and (719 253 ± 376 519) gray scale area × mg⁻¹ × L⁻¹. Both in cerebral aneurysm group were significantly higher than that in control group (P < 0.05) . The TNOS and iNOS levels in cerebral aneurysm group were (23.6 ± 6.6) and (11.4 ± 2.6) U/mgprot. The difference of TNOS level was not significant between aneurysm and control groups (P > 0.05) while the levels of iNOS and iNOS/TNOS in cerebral aneurysm group were significantly higher than that in control group (P < 0.05).

CONCLUSION

MMP-2, MMP-9 and iNOS are closely correlated with cerebral aneurysm.

摘要

目的

探讨基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和诱导型一氧化氮合酶（iNOS）在脑动脉瘤中的表达情况，并与正常脑血管组织进行比较，以更好地了解脑动脉瘤的发病机制。

方法

2009年至2012年期间，在内蒙古自治区人民医院和北京天坛医院，手术中获取12例脑动脉瘤样本，并取10例颞叶癫痫手术中的皮质动脉作为对照。采用分光光度法和底物凝胶酶谱法检测标本中MMP-2、MMP-9和iNOS的活性。

结果

脑动脉瘤组MMP-2和MMP-9水平分别为（199 598±125 288）灰度面积×mg⁻¹×L⁻¹和（719 253±376 519）灰度面积×mg⁻¹×L⁻¹。两者在脑动脉瘤组均显著高于对照组（P<0.05）。脑动脉瘤组总一氧化氮合酶（TNOS）和诱导型一氧化氮合酶（iNOS）水平分别为（23.6±6.6）和（11.4±2.6）U/mgprot。动脉瘤组与对照组TNOS水平差异无统计学意义（P>0.05），而脑动脉瘤组iNOS及iNOS/TNOS水平显著高于对照组（P<0.05）。