Dent G, Ukena D, Sybrecht G W, Barnes P J
Department of Thoracic Medicine, National Heart and Lung Institute, London, U.K.
Eur J Pharmacol. 1989 Oct 10;169(2-3):313-6. doi: 10.1016/0014-2999(89)90029-0.
The radiolabelled platelet activating factor (PAF) receptor antagonist, [3H]WEB 2086, bound to specific sites on membrane fractions from homogenised guinea pig and human lungs. The sites on guinea pig and human membranes bound [3H]WEB 2086 with dissociation constants (KD) of 16.8 and 22.6 nM and binding capacities (Bmax) of 203 and 157 fmol/mg protein, respectively. In both species, binding was displaced competitively by PAF, suggesting that the sites labelled by [3H]WEB 2086 are PAF receptors.
放射性标记的血小板活化因子（PAF）受体拮抗剂[3H]WEB 2086与豚鼠和人肺匀浆膜组分上的特异性位点结合。豚鼠和人膜上的位点与[3H]WEB 2086结合，解离常数（KD）分别为16.8和22.6 nM，结合容量（Bmax）分别为203和157 fmol/mg蛋白。在这两个物种中，PAF竞争性地取代了结合，这表明[3H]WEB 2086标记的位点是PAF受体。
