Liu Junjun, Liu Xiaozhen, Feng Xiaolong, Liu Jian, Lv Shuhua, Zhang Wei, Niu Yun
Department of Breast Cancer Pathology and Research Laboratory, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, West Huanhu Road, Ti Yuan Bei, Hexi District, Tianjin, 300060, China.
Breast Cancer Res Treat. 2015 Jan;149(2):395-401. doi: 10.1007/s10549-014-3214-1. Epub 2014 Dec 23.
We determined c-kit expression in the stroma and epithelia of benign, borderline, and malignant phyllodes tumors (PTs), respectively, as well as the relationship between c-kit expression in stromal elements and KIT gene copy number variations (CNVs). To assess c-kit expression and KIT CNVs, 348 PT cases were studied: 120 (34.4 %) benign cases, 115 (33.1 %) borderline cases, and 113 (32.5 %) malignant cases. All of these cases were evaluated for c-kit (CD117) expression using immunohistochemistry. Forty-two cases (29 c-kit-positive in the stromal cells cases and 13 negative cases) were investigated for KIT gene CNVs via genomic polymerase chain reaction (PCR). The overall rate of c-kit positivity in the stroma was 46.8 %, as well as 24.2, 53.1, and 64.6 %, respectively, in PTs of three different grades. However, in the majority of cases, the epithelia were c-kit positive (98.2 %), and the positivity was 100, 99.1, and 95 % in PTs of three different grades, respectively. There was a significant change in the expression of c-kit in the stroma and epithelia according to grade (P < 0.001, P = 0.014). From the genomic PCR results, we can confirm that c-kit positivity in the stroma is directly correlated with KIT gene copy numbers increases (P = 0.003, P = 0.041). We demonstrated that c-kit expression in the stroma of PTs is positively associated with malignancy. c-Kit epithelial positivity was inversely correlated with PTs malignancy. c-Kit overexpression in the stroma was related to KIT gene copy numbers increases.
我们分别测定了良性、交界性和恶性叶状肿瘤（PTs）的基质和上皮中c-kit的表达情况，以及基质成分中c-kit表达与KIT基因拷贝数变异（CNVs）之间的关系。为评估c-kit表达和KIT CNVs，研究了348例PT病例：120例（34.4%）良性病例、115例（33.1%）交界性病例和113例（32.5%）恶性病例。所有这些病例均采用免疫组织化学法评估c-kit（CD117）表达。通过基因组聚合酶链反应（PCR）对42例病例（29例基质细胞c-kit阳性病例和13例阴性病例）进行KIT基因CNVs检测。基质中c-kit阳性的总体率为46.8%，在三种不同分级的PTs中分别为24.2%、53.1%和64.6%。然而，在大多数病例中，上皮c-kit呈阳性（98.2%），在三种不同分级的PTs中阳性率分别为100%、99.1%和95%。根据分级，基质和上皮中c-kit的表达有显著变化（P<0.001，P = 0.014）。从基因组PCR结果可知，基质中c-kit阳性与KIT基因拷贝数增加直接相关（P = 0.003，P = 0.041）。我们证明PTs基质中c-kit表达与恶性程度呈正相关。c-Kit上皮阳性与PTs恶性程度呈负相关。基质中c-Kit过表达与KIT基因拷贝数增加有关。
