从猪肾中免疫亲和分离钠钾ATP酶α3亚型

Immunoaffinity isolation of Na,K-ATPase alpha 3 isoform from pig kidney.

作者信息

Arystarkhova E A, Lakhtina O E, Levina N B, Modyanov N N

机构信息

Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1989 Oct 23;257(1):24-6. doi: 10.1016/0014-5793(89)81776-4.

DOI:10.1016/0014-5793(89)81776-4

PMID:2553492

Abstract

The Na,K-ATPase alpha 3 isoform of the catalytic subunit has been isolated from pig kidney microsomes. The procedure employs immunoaffinity chromatography on Sepharose 4B covalently coupled with monospecific antibodies a-II against the synthetic peptide including the putative alpha 3 N-terminus. The structural analysis provides unambiguous proof that the isolated protein corresponds to the third transcript for the alpha 3 isoform. The N-terminal amino acid sequence determined. Met-Gly-Asp-Lys-Lys-Asp-Asp, shows that unlike the alpha 1 and alpha 2 proteins, the mature Na,K-ATPase isoform lacks post-translational proteolytic processing.

摘要

已从猪肾微粒体中分离出催化亚基的钠钾ATP酶α3亚型。该方法采用在共价偶联有针对包含假定α3 N端的合成肽的单特异性抗体a-II的琼脂糖4B上进行免疫亲和层析。结构分析提供了明确的证据，证明分离出的蛋白质对应于α3亚型的第三种转录本。所确定的N端氨基酸序列为Met-Gly-Asp-Lys-Lys-Asp-Asp，表明与α1和α2蛋白不同，成熟的钠钾ATP酶亚型缺乏翻译后蛋白水解加工。