Multiple high-frequency transpositions of Tn5 in Serratia marcescens 274 from a thermosensitive replication mutant of plasmid R388.
作者信息
Estepa G R, Harshey R M
机构信息
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
出版信息
Gene. 1989 Sep 30;81(2):355-9. doi: 10.1016/0378-1119(89)90196-0.
PMID:2553541
Abstract
We have used the broad-host-range conjugative suicide plasmid vector described by Sasakawa and Yoshikawa [Gene 56 (1987) 283-288] for transposon mutagenesis of Serratia marcescens 274. We report multiple transposition events of Tn5 from this vector. In addition, the unusual pattern of Tn5 transposition might provide an insight into its regulation.
摘要
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