A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis.
作者信息
Sasakawa C, Yoshikawa M
机构信息
Institute of Medical Science, University of Tokyo, Japan.
出版信息
Gene. 1987;56(2-3):283-8. doi: 10.1016/0378-1119(87)90145-4.
PMID:2824292
Abstract
A series of variants of transposon Tn5 were constructed by replacement of the 2.7-kb central segment which encodes kanamycin resistance with various other resistance-coding genes: tetracycline, chloramphenicol, gentamicin, trimethoprim, streptomycin or ampicillin. A thermosensitive replication mutant of the broad-host-range transmissible plasmid R388 was also constructed for use as a suicide vector for the delivery of transposable elements.
摘要
Zotero 插件