细胞色素c电化学在超氧自由基产生量测定中的应用。

Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production.

作者信息

McNeil C J, Smith K A, Bellavite P, Bannister J V

机构信息

Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, England.

出版信息

Free Radic Res Commun. 1989;7(2):89-96. doi: 10.3109/10715768909087928.

DOI:10.3109/10715768909087928

PMID:2553552

Abstract

An electrochemical sensor for the measurement of the superoxide anion produced as a result of the respiratory burst of neutrophils is described. The reduction of cytochrome c by the superoxide ion was monitored using a surface-modified gold electrode. The current produced was shown to be superoxide-specific and proportional to the number of neutrophils in both purified neutrophil preparations and plasma.

摘要

描述了一种用于测量嗜中性粒细胞呼吸爆发产生的超氧阴离子的电化学传感器。使用表面改性的金电极监测超氧离子对细胞色素c的还原作用。结果表明，所产生的电流具有超氧特异性，并且与纯化的嗜中性粒细胞制剂和血浆中的嗜中性粒细胞数量成正比。