Tammeveski K, Tenno T T, Mashirin A A, Hillhouse E W, Manning P, McNeil C J
Institute of Physical Chemistry, University of Tartu, Estonia.
Free Radic Biol Med. 1998 Nov 15;25(8):973-8. doi: 10.1016/s0891-5849(98)00182-8.
We have recently described an optimised electrode for the detection of enzymatic and cellular superoxide (O2*-) production based on cytochrome c immobilized covalently at a surface-modified gold electrode and applied this system to the study of free radical production by activated human glioblastoma cells. In this paper we report the development of a mathematical model for the O2*- electrode responding to enzymically produced O2*- which should enable the determination of absolute concentrations of O2*- in biological systems when this electrode is employed for direct, real-time monitoring of free radical release and interactions.
我们最近描述了一种用于检测酶促和细胞超氧化物（O2*-）生成的优化电极，该电极基于共价固定在表面修饰金电极上的细胞色素c，并将此系统应用于研究活化的人胶质母细胞瘤细胞产生自由基的情况。在本文中，我们报告了一种针对响应酶促产生的O2*-的O2*-电极的数学模型的开发，当该电极用于直接实时监测自由基释放和相互作用时，该模型应能够测定生物系统中O2*-的绝对浓度。
