Lin Ze Bang, Qian Bo, Yang Yu Zhong, Zhou Kai, Sun Jian, Mo Xu Ming, Wu Kai Hong
Department of Cardiothoracic Surgery, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, China.
J Cell Biochem. 2015 Jul;116(7):1205-12. doi: 10.1002/jcb.25072.
Mesenchymal stromal cells (MSCs) are promising candidate donor cells for replacement of cardiomyocyte loss during ischemia and in vitro generation of myocardial tissue. We have successfully isolated MSCs from the discarded neonatal thymus gland during cardiac surgery. The thymus MSCs were characterized by cell-surface antigen expression. These cells have high ability for proliferation and are able to differentiate into osteoblasts and adipocytes in vitro. For cardiac differentiation, the cells were divided into 3 groups: untreated control; 5-azacytidine group and sequential exposure to 5-azacytidine, bone morphogenetic protein 4, and basic fibroblast growth factor. Thymus MSCs showed a fibrolast-like morphology and some differentiated cells increased in size, formed a ball-like appearance over time and spontaneously contracting cells were observed in sequential exposure group. Immunostaining studies, cardiac specific genes/protein expression confirmed the cardiomyocyte phenotype of the differentiated cells. These results demonstrate that thymus MSCs can be a promising cellular source for cardiac cell therapy and tissue engineering.
间充质基质细胞（MSCs）是缺血期间替代心肌细胞损失以及体外生成心肌组织的有前景的候选供体细胞。我们已成功在心脏手术期间从废弃的新生儿胸腺中分离出MSCs。胸腺MSCs通过细胞表面抗原表达进行表征。这些细胞具有高增殖能力，并且能够在体外分化为成骨细胞和脂肪细胞。对于心脏分化，将细胞分为3组：未处理的对照组；5-氮杂胞苷组以及依次暴露于5-氮杂胞苷、骨形态发生蛋白4和碱性成纤维细胞生长因子组。胸腺MSCs呈现出成纤维细胞样形态，一些分化细胞体积增大，随着时间的推移形成球状外观，并且在依次暴露组中观察到自发收缩的细胞。免疫染色研究、心脏特异性基因/蛋白表达证实了分化细胞的心肌细胞表型。这些结果表明胸腺MSCs可以成为心脏细胞治疗和组织工程的有前景的细胞来源。
