Wang Bin, Guo Cunlan, Lou Zhichao, Xu Bingqian
Single Molecule Study Laboratory, Faculty of Engineering and Nanoscale Science and Engineering Center, University of Georgia, Athens, GA 30602, USA.
Chem Commun (Camb). 2015 Feb 7;51(11):2088-90. doi: 10.1039/c4cc09209k. Epub 2014 Dec 23.
Aggregations of human prion protein (23-231) were monitored by atomic force microscopy in real-time under pH 4. Prion dimers and trimers were determined as the basic units by AFM images and simulated structures. Aggregates aligned with the herringbone structures of an Au(111) reconstructed surface via Au-S bonds as the first layer, while the second layer was formed by non-covalent interactions.
在pH 4条件下，通过原子力显微镜实时监测人朊病毒蛋白（23 - 231）的聚集体。通过原子力显微镜图像和模拟结构确定朊病毒二聚体和三聚体为基本单元。聚集体通过金硫键作为第一层与金（111）重构表面的人字形结构对齐，而第二层则由非共价相互作用形成。
