Salm A K, McCarthy K D
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599.
Glia. 1989;2(5):346-52. doi: 10.1002/glia.440020507.
Astrocytes are the most numerous cell type in the central nervous system. When cultured, these cells express a wide variety of receptors for neurotransmitters. Nonetheless, it has yet to be firmly established that adult astrocytes in situ express receptors for neurotransmitters. In this report the technique of combined receptor autoradiography and immunocytochemistry has been applied to isolated astrocytes from adult animals to examine beta-adrenergic receptor (beta-AR) expression by these cells. It is shown that fibrous and protoplasmic astrocytes isolated from adult rat cerebral cortices specifically bind the beta-AR ligand iodocyanopindolol and that this binding is inhibited by the beta-AR ligands propranolol and isoproterenol. These results indicate that at least two of the major morphological subtypes of adult astrocytes express beta-ARs.
星形胶质细胞是中枢神经系统中数量最多的细胞类型。在培养时，这些细胞表达多种神经递质受体。然而，成年星形胶质细胞在原位是否表达神经递质受体尚未得到确凿证实。在本报告中，受体放射自显影术和免疫细胞化学相结合的技术已应用于从成年动物分离出的星形胶质细胞，以检测这些细胞中β-肾上腺素能受体（β-AR）的表达。结果表明，从成年大鼠大脑皮层分离出的纤维型和原浆型星形胶质细胞能特异性结合β-AR配体碘氰吲哚洛尔，且这种结合可被β-AR配体普萘洛尔和异丙肾上腺素抑制。这些结果表明，成年星形胶质细胞的至少两种主要形态亚型表达β-ARs。
