González Oscar, Iglesias Carmela, Zafon Carles, Castellví Josep, García-Burillo Amparo, Temprana Jordi, Caubet Enric, Vilallonga Ramón, Mesa Jordi, Ramón y Cajal Santiago, Fort José Manuel, Armengol Manel, Balibrea José María
Department of General Surgery, Endocrine Surgery Division.
J Invest Surg. 2015 Jun;28(3):153-9. doi: 10.3109/08941939.2014.990123. Epub 2014 Dec 23.
Purpouse: One Step Nucleic Acid Amplification (OSNA) has been previously proposed for the diagnosis of lymph node metastases (LNMs) from several malignant conditions by quantifying the number of copies of cytokeratin 19 mRNA. Our aim was to evaluate the results obtained by OSNA in the lymph nodes of patients with papillary thyroid carcinoma (PTC) by comparing our results with the findings observed using standard pathological examination.
Fifty human lymph nodes (from five patients with diagnosed PTC) were studied. Each node was divided into two: one half was used for molecular study ("OSNA-node"), and the other half was used for conventional staining with hematoxylin and eosin ("HE-non-OSNA node"). Three cytological imprints using Papanicolaou and May-Grunwald-Giemsa strains were obtained from both node halves. The results from each technique were compared, and ROC analysis was performed.
The OSNA study showed 22 positive samples for LNM (44%), which demonstrate a high concordance rate with the results observed using conventional pathological examination (cytology of "OSNA-node" and HE of "Non-OSNA node") with specificity and sensitivity values greater than 86% and 89%, respectively. However, both comparisons differed in the number of copies of mRNA as the best cut-off (260 copies in the first case and 93 in the second case).
The OSNA results for the detection of LNM in patients with PTC are comparable with those observed using conventional techniques. However, its quantitative nature could be useful to more accurately detect lymph node involvement.
目的：先前已提出一步核酸扩增（OSNA）技术，通过定量细胞角蛋白19 mRNA的拷贝数来诊断多种恶性疾病的淋巴结转移（LNM）。我们的目的是通过将我们的结果与标准病理检查的结果进行比较，评估OSNA技术在甲状腺乳头状癌（PTC）患者淋巴结中的应用结果。
研究了50个人类淋巴结（来自5例确诊为PTC的患者）。每个淋巴结被分成两半：一半用于分子研究（“OSNA淋巴结”），另一半用于苏木精和伊红常规染色（“HE非OSNA淋巴结”）。从两半淋巴结中均获取了三张使用巴氏染色法和迈-格-姬染色法的细胞学印片。比较了每种技术的结果，并进行了ROC分析。
OSNA研究显示22个LNM阳性样本（44%），其与常规病理检查（“OSNA淋巴结”的细胞学检查和“非OSNA淋巴结”的HE染色）结果具有较高的一致性，特异性和敏感性值分别大于86%和89%。然而，作为最佳临界值的mRNA拷贝数在两种比较中有所不同（第一种情况为260拷贝，第二种情况为93拷贝）。
OSNA技术检测PTC患者LNM的结果与常规技术相当。然而，其定量特性可能有助于更准确地检测淋巴结受累情况。
