De Kretser D M, O'Leary P C, Irby D C, Risbridger G P
Department of Anatomy, Monash University, Melbourne, Australia.
Int J Androl. 1989 Aug;12(4):273-80. doi: 10.1111/j.1365-2605.1989.tb01314.x.
Adult male rats given a single intraperitoneal injection of the Leydig cell cytotoxin ethane dimethane sulphonate (EDS) show a significant decrease in testosterone from 7 to 14 days, and elevation of serum FSH and LH levels commencing 7 days after treatment, returning to normal at 28 days for LH and 49 days for FSH. A significant rise in serum inhibin levels was seen at day 14 after EDS treatment with levels returning to normal at day 49. In a second series of experiments, silastic implants of testosterone, either 2.5 cm or 22.5 cm in length, were introduced subcutaneously into adult male rats which were treated with EDS 10 days later. Both doses of testosterone suppressed basal LH levels but did not significantly change FSH levels. The rise in FSH and LH levels seen in normal rats after EDS treatment did not occur in either group of testosterone-implanted rats. However, serum inhibin levels rose significantly in both groups after EDS treatment, suggesting that the rise in serum inhibin levels was not due to stimulation arising from the increase in FSH levels after EDS treatment. The data suggest that the rise in serum inhibin levels after EDS treatment is linked to destruction of the Leydig cells through mechanisms that require further investigation.
成年雄性大鼠腹腔注射一次莱迪希细胞细胞毒素乙烷二甲磺酸盐（EDS）后，睾酮水平在7至14天显著下降，血清促卵泡激素（FSH）和促黄体生成素（LH）水平在治疗7天后开始升高，LH在28天恢复正常，FSH在49天恢复正常。EDS治疗后第14天血清抑制素水平显著升高，第49天恢复正常。在第二系列实验中，将长度为2.5厘米或22.5厘米的睾酮硅橡胶植入物皮下植入成年雄性大鼠体内，10天后对其进行EDS治疗。两种剂量的睾酮均抑制基础LH水平，但对FSH水平无显著影响。在接受EDS治疗的正常大鼠中观察到的FSH和LH水平升高在两组植入睾酮的大鼠中均未出现。然而，EDS治疗后两组大鼠的血清抑制素水平均显著升高，这表明血清抑制素水平的升高并非由于EDS治疗后FSH水平升高所引起的刺激。数据表明，EDS治疗后血清抑制素水平的升高与莱迪希细胞的破坏有关，其机制有待进一步研究。
