Ruyra Angels, Torrealba Debora, Morera Davinia, Tort Lluís, MacKenzie Simon, Roher Nerea
Institut de Biotecnologia i de Biomedicina - Parc de Recerca UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Institut de Biotecnologia i de Biomedicina - Parc de Recerca UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain; Departament de Biologia Cel·lular, Fisiologia Animal i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Comp Biochem Physiol B Biochem Mol Biol. 2015 Apr;182:55-63. doi: 10.1016/j.cbpb.2014.12.002. Epub 2014 Dec 20.
The zebrafish (Danio rerio) is a widely used model species for biomedical research and is also starting to be a model for aquaculture research. The ZFL cell line, established from zebrafish liver, has been mostly used in toxicological and ecotoxicological studies. However, no studies have previously characterised this cell line in regard to its immunological response. The aim of this work was to study the gene expression response of the ZFL cell line after incubation with different prototypical immune stimuli, such as lipopolysaccharide (LPS), peptidoglycan (PGN), zymosan, and with a special focus on the dsRNA Poly (I:C). Using PCR, microarrays, and confocal microscopy we have explored the response of the ZFL cells against Poly (I:C). This study shows that the ZFL is able to uptake very efficiently the Poly (I:C) and mount a strong anti-viral response. We can conclude that ZFL could be used not only in toxicological studies, but also in studying anti-viral responses in zebrafish.
斑马鱼（Danio rerio）是生物医学研究中广泛使用的模式物种，也开始成为水产养殖研究的模式生物。从斑马鱼肝脏建立的ZFL细胞系主要用于毒理学和生态毒理学研究。然而，此前尚无关于该细胞系免疫反应特征的研究。这项工作的目的是研究ZFL细胞系在与不同的典型免疫刺激物（如脂多糖（LPS）、肽聚糖（PGN）、酵母聚糖）孵育后的基因表达反应，特别关注双链RNA聚肌苷酸胞苷酸（Poly (I:C)）。我们使用聚合酶链反应（PCR）、微阵列和共聚焦显微镜研究了ZFL细胞对Poly (I:C)的反应。这项研究表明，ZFL能够非常有效地摄取Poly (I:C)并引发强烈的抗病毒反应。我们可以得出结论，ZFL不仅可用于毒理学研究，还可用于研究斑马鱼的抗病毒反应。
