Dirican Ahmed, Erten Cigdem, Atmaca Harika, Bozkurt Emir, Kucukzeybek Yuksel, Varol Umut, Oktay Tarhan Mustafa, Karaca Burcak, Uslu Ruchan
Medical Oncology Clinic, Izmir Katip Celebi University Ataturk Training and Research Hospital, Izmir, Turkey.
J BUON. 2014 Oct-Dec;19(4):1055-61.
Thymoquinone (TQ), an active ingredient of black seed oil (Nigella Sativa), has been shown to possess cytotoxic activity against a variety of cancer cell lines. Our purpose was to investigate if the cytotoxic and apoptotic effect of zoledronic acid (ZA) can be enhanced by the addition of the TQ in hormone- and drug-refractory prostate cancer cells PC-3 and DU-145.
XTT cell proliferation assay was used to assess cytotoxicity; DNA fragmentation and caspase 3/7 activity were also measured.
The combination of TQ and ZA resulted in a significant synergistic cytotoxic activity and DNA fragmentation when compared to any single agent alone, in a dose- and time-dependent manner. In addition, TQ and ZA combination increased the caspase 3/7 activity in PC-3 cell line, while this activity could not be demonstrated in DU-145 cell line.
TQ and ZA had minimal hematological and non-hematological toxicity profile compared to cytotoxic agents. So, this combination may be an alternative approach for patients who are unable to be treated by conventional treatments because of poor performance status.
黑种草籽（Nigella Sativa）中的活性成分百里醌（TQ）已显示出对多种癌细胞系具有细胞毒性活性。我们的目的是研究在激素难治性和药物难治性前列腺癌细胞PC-3和DU-145中添加TQ是否能增强唑来膦酸（ZA）的细胞毒性和凋亡作用。
采用XTT细胞增殖试验评估细胞毒性；还测量了DNA片段化和半胱天冬酶3/7活性。
与单独使用任何一种药物相比，TQ和ZA的组合以剂量和时间依赖性方式产生了显著的协同细胞毒性活性和DNA片段化。此外，TQ和ZA组合增加了PC-3细胞系中的半胱天冬酶3/7活性，而在DU-145细胞系中未显示出这种活性。
与细胞毒性药物相比，TQ和ZA的血液学和非血液学毒性最小。因此，对于因身体状况不佳而无法接受传统治疗的患者，这种联合治疗可能是一种替代方法。
