Grandin Clément, Lucas-Hourani Marianne, Clavel Marine, Taborik Fabrice, Vabret Astrid, Tangy Frédéric, Contamin Hugues, Vidalain Pierre-Olivier
CNRS, UMR3569, Paris, France.
Institut Pasteur, Unité de Génomique Virale et Vaccination, Paris, France.
J Gen Virol. 2015 Apr;96(Pt 4):782-792. doi: 10.1099/vir.0.000039. Epub 2014 Dec 23.
There is no large-scale therapy available against human respiratory syncytial virus (hRSV), a major pathogen responsible for acute respiratory diseases. Macaques represent an interesting animal model to evaluate potential treatments because of their genetic, anatomical and immunological proximity with humans. However, the parameters that influence hRSV growth and control in this model are still poorly understood. We have documented in the following study the influence of age as well as repeated infections on the virological, clinical and immunological parameters of this animal model. Following intranasal inoculation, hRSV replicated in the upper respiratory tract for less than 15 days with no clinical signs regardless of age. Interestingly, we observed the induction of a local immune response at the nasal mucosa as assessed by expression profiles of inflammatory and IFN-stimulated genes. Animals also developed specific antibodies and were immune to reinfection. Thus, we showed that even in infant macaques, intranasal hRSV infection induced both local and systemic immune responses to efficiently control the virus.
目前尚无针对人类呼吸道合胞病毒（hRSV）的大规模治疗方法，hRSV是引起急性呼吸道疾病的主要病原体。由于猕猴在基因、解剖结构和免疫方面与人类相近，因此是评估潜在治疗方法的有趣动物模型。然而，在该模型中影响hRSV生长和控制的参数仍知之甚少。在以下研究中，我们记录了年龄以及重复感染对该动物模型的病毒学、临床和免疫学参数的影响。经鼻内接种后，无论年龄大小，hRSV在上呼吸道中复制不到15天，且无临床症状。有趣的是，通过炎症和IFN刺激基因的表达谱评估，我们观察到鼻黏膜处诱导了局部免疫反应。动物还产生了特异性抗体，并对再次感染具有免疫力。因此，我们表明，即使在幼年猕猴中，经鼻内hRSV感染也会诱导局部和全身免疫反应，从而有效控制病毒。
