蛋白质在四膜虫大核和小核中的细胞核特异性及时间限制定位。
Nucleus-specific and temporally restricted localization of proteins in Tetrahymena macronuclei and micronuclei.
作者信息
White E M, Allis C D, Goldfarb D S, Srivastva A, Weir J W, Gorovsky M A
机构信息
Department of Biology, University of Rochester, New York 14627.
出版信息
J Cell Biol. 1989 Nov;109(5):1983-92. doi: 10.1083/jcb.109.5.1983.
Abstract
Labeled nuclear proteins were microinjected into the cytoplasm of Tetrahymena thermophila. Macronuclear H1, calf thymus H1, and the SV40 large T antigen nuclear localization signal linked to BSA accumulated specifically in macronuclei, even if cells were in micronuclear S phase or were nonreplicating. The way in which histone H4 localized to either the macronucleus or the micronucleus suggested that it accumulates in whichever nucleus is replicating. The inability of the micronucleus to accumulate Tetrahymena H1 or heterologous nuclear proteins, even at a period in the cell cycle when it is accumulating H4, suggests that it has a specialized transport system. These studies demonstrate that although the mechanism for localizing proteins to nuclei is highly conserved among eukaryotes, it can differ between two porecontaining nuclei lying in the same cytoplasm.
摘要
将标记的核蛋白显微注射到嗜热四膜虫的细胞质中。即使细胞处于微核S期或不进行复制，大核H1、小牛胸腺H1以及与牛血清白蛋白相连的SV40大T抗原核定位信号也会特异性地积累在大核中。组蛋白H4定位到大核或微核的方式表明，它会在正在进行复制的任何一个核中积累。即使在细胞周期中微核积累H4的时期，微核也无法积累嗜热四膜虫H1或异源核蛋白，这表明它具有专门的转运系统。这些研究表明，尽管真核生物中蛋白质定位到细胞核的机制高度保守，但位于同一细胞质中的两个含孔细胞核之间的机制可能不同。
相似文献
1
Nucleus-specific and temporally restricted localization of proteins in Tetrahymena macronuclei and micronuclei.蛋白质在四膜虫大核和小核中的细胞核特异性及时间限制定位。
J Cell Biol. 1989 Nov;109(5):1983-92. doi: 10.1083/jcb.109.5.1983.
2
Histone rearrangements accompany nuclear differentiation and dedifferentiation in Tetrahymena.组蛋白重排伴随着四膜虫的细胞核分化和去分化过程。
Dev Biol. 1984 Feb;101(2):282-94. doi: 10.1016/0012-1606(84)90142-8.
3
Nuclear localization signal targeting to macronucleus and micronucleus in binucleated ciliate Tetrahymena thermophila.核定位信号靶向双核嗜热四膜虫大核和小核
Genes Cells. 2018 Jul;23(7):568-579. doi: 10.1111/gtc.12602. Epub 2018 Jun 8.
4
Modulation of linker histones during development in Tetrahymena: selective elimination of linker histone during the differentiation of new macronuclei.嗜热四膜虫发育过程中连接组蛋白的调控：新大核分化过程中连接组蛋白的选择性消除。
Dev Biol. 1985 May;109(1):1-8. doi: 10.1016/0012-1606(85)90339-2.
5
Timing of the appearance of macronuclear-specific histone variant hv1 and gene expression in developing new macronuclei of Tetrahymena thermophila.嗜热四膜虫发育中新大核中巨核特异性组蛋白变体hv1的出现时间及基因表达
J Cell Biol. 1984 Jun;98(6):2107-17. doi: 10.1083/jcb.98.6.2107.
6
Timing of the appearance of ubiquitinated histones in developing new macronuclei of Tetrahymena thermophila.嗜热四膜虫发育中新大核中泛素化组蛋白出现的时间。
Biochem Cell Biol. 1991 Jan;69(1):66-71. doi: 10.1139/o91-009.
7
Cell-cycle regulation as a mechanism for targeting proteins to specific DNA sequences in Tetrahymena thermophila.细胞周期调控作为嗜热四膜虫中蛋白质靶向特定DNA序列的一种机制。
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2205-9. doi: 10.1073/pnas.85.7.2205.
8
Histone acetylation in conjugating Tetrahymena thermophila.嗜热四膜虫接合过程中的组蛋白乙酰化
J Cell Biol. 1989 Sep;109(3):1007-14. doi: 10.1083/jcb.109.3.1007.
9
Proteolytic removal of core histone amino termini and dephosphorylation of histone H1 correlate with the formation of condensed chromatin and transcriptional silencing during Tetrahymena macronuclear development.在四膜虫大核发育过程中，核心组蛋白氨基末端的蛋白水解去除以及组蛋白H1的去磷酸化与浓缩染色质的形成和转录沉默相关。
Genes Dev. 1991 Sep;5(9):1601-10. doi: 10.1101/gad.5.9.1601.
10
The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus.嗜热四膜虫的CNA1组蛋白对于生殖系小核中的染色体分离至关重要。
Mol Biol Cell. 2006 Jan;17(1):485-97. doi: 10.1091/mbc.e05-07-0698. Epub 2005 Oct 26.
引用本文的文献
1
Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena.核孔复合体的偏向性组装是四膜虫体细胞和生殖细胞核分化所必需的。
J Cell Sci. 2015 May 1;128(9):1812-23. doi: 10.1242/jcs.167353. Epub 2015 Mar 18.
2
Identification and localisation of a nucleoporin-like protein component of the plant nuclear matrix.鉴定和定位植物核基质中的核孔蛋白样蛋白成分。
Planta. 1992 Jun;187(3):414-20. doi: 10.1007/BF00195666.
3
Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes.嗜热四膜虫，一种具有独立生殖系和体细胞基因组的单细胞真核生物。
Res Microbiol. 2011 Jul-Aug;162(6):578-86. doi: 10.1016/j.resmic.2011.05.001. Epub 2011 May 18.
4
Regulation of nucleocytoplasmic transport in skeletal muscle.核质转运在骨骼肌中的调控。
Curr Top Dev Biol. 2011;96:273-302. doi: 10.1016/B978-0-12-385940-2.00010-3.
5
Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila.细胞核特异性输入蛋白α和核孔蛋白调节双核嗜热四膜虫中的蛋白质输入和细胞核分裂。
Eukaryot Cell. 2008 Sep;7(9):1487-99. doi: 10.1128/EC.00193-08. Epub 2008 Aug 1.
6
An abundant nucleolar phosphoprotein is associated with ribosomal DNA in Tetrahymena macronuclei.一种丰富的核仁磷蛋白与嗜热四膜虫大核中的核糖体DNA相关联。
Mol Biol Cell. 1997 Jan;8(1):97-108. doi: 10.1091/mbc.8.1.97.
7
An abundant high-mobility-group-like protein is targeted to micronuclei in a cell cycle-dependent and developmentally regulated fashion in Tetrahymena thermophila.在嗜热四膜虫中，一种丰富的高迁移率族样蛋白以细胞周期依赖性和发育调控的方式定位于微核。
Mol Cell Biol. 1993 Jan;13(1):163-73. doi: 10.1128/mcb.13.1.163-173.1993.
8
Four distinct and unusual linker proteins in a mitotically dividing nucleus are derived from a 71-kilodalton polyprotein, lack p34cdc2 sites, and contain protein kinase A sites.在有丝分裂的细胞核中，四种不同寻常的连接蛋白源自一种71千道尔顿的多蛋白，缺乏p34cdc2位点，并含有蛋白激酶A位点。
Mol Cell Biol. 1994 Jan;14(1):10-20. doi: 10.1128/mcb.14.1.10-20.1994.
9
Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway.核运输中的多种途径：U2 小核核糖核蛋白的输入通过一种新的动力学途径发生。
J Cell Biol. 1991 Jan;112(2):215-23. doi: 10.1083/jcb.112.2.215.
10
Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.核蛋白的输入受到一种针对核孔复合体糖蛋白腔内表位的抗体的抑制。
J Cell Biol. 1992 Jan;116(1):15-30. doi: 10.1083/jcb.116.1.15.
本文引用的文献
1
Cytochemical studies on the problem of macronuclear subnuclei in tetrahymena.关于四膜虫大核亚核问题的细胞化学研究。
Genetics. 1972 Feb;70(2):251-60. doi: 10.1093/genetics/70.2.251.
2
Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.在分裂细胞中，基础组蛋白合成与S期组蛋白合成的分离。
Cell. 1981 Dec;27(2 Pt 1):321-30. doi: 10.1016/0092-8674(81)90415-3.
3
Regulation of histone acetylation in Tetrahymena macro- and micronuclei.嗜热四膜虫大核和小核中组蛋白乙酰化的调控
J Biol Chem. 1982 Mar 10;257(5):2591-8.
4
Proteolytic processing of histone H3 in chromatin: a physiologically regulated event in Tetrahymena micronuclei.染色质中组蛋白H3的蛋白水解加工：四膜虫微核中的一种生理调节事件。
Cell. 1980 May;20(1):55-64. doi: 10.1016/0092-8674(80)90234-2.
5
Proteolytic processing of micronuclear H3 and histone phosphorylation during conjugation in Tetrahymena thermophila.嗜热四膜虫接合期间微核H3的蛋白水解加工及组蛋白磷酸化
Exp Cell Res. 1984 Aug;153(2):287-98. doi: 10.1016/0014-4827(84)90601-3.
6
Germ line-specific DNA sequences are present on all five micronuclear chromosomes in Tetrahymena thermophila.种系特异性DNA序列存在于嗜热四膜虫的所有五条微核染色体上。
Mol Cell Biol. 1983 Nov;3(11):1909-19. doi: 10.1128/mcb.3.11.1909-1919.1983.
7
Two-dimensional gel analysis of the fate of oocyte nuclear proteins in the development of Xenopus laevis.非洲爪蟾发育过程中卵母细胞核蛋白命运的二维凝胶分析。
Dev Biol. 1983 Dec;100(2):412-25. doi: 10.1016/0012-1606(83)90235-x.
8
Accumulation of the isolated carboxy-terminal domain of histone H1 in the Xenopus oocyte nucleus.组蛋白H1分离的羧基末端结构域在非洲爪蟾卵母细胞核中的积累。
EMBO J. 1984 Sep;3(9):1933-7. doi: 10.1002/j.1460-2075.1984.tb02072.x.
9
Proteins shifting from the cytoplasm into the nuclei during early embryogenesis of Drosophila melanogaster.在黑腹果蝇的早期胚胎发育过程中，蛋白质从细胞质转移到细胞核。
Dev Biol. 1984 Jul;104(1):37-48. doi: 10.1016/0012-1606(84)90034-4.
10
Heat shock and deciliation induce phosphorylation of histone H1 in T. pyriformis.热休克和去纤毛作用诱导梨形四膜虫中组蛋白H1的磷酸化。
Cell. 1981 Jan;23(1):73-7. doi: 10.1016/0092-8674(81)90271-3.
Zotero 插件