墨西哥尤卡坦半岛三个城市野生哺乳动物中猫立克次体的检测
Detection of Rickettsia felis in Wild Mammals from Three Municipalities in Yucatan, Mexico.
作者信息
Panti-May Jesús Alonso, Torres-Castro Marco, Hernández-Betancourt Silvia, Dzul-Rosado Karla, Zavala-Castro Jorge, López-Avila Karina, Tello-Martín Raúl
机构信息
PIMVS Tumben Kuxtal A.C., Calle 12 #64 x 5 y 7, C.P. 97470, Tixkokob, Yucatán, Mexico.
Departamento de Zoología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 carretera Mérida-Xmatkuil, C.P. 97315, Mérida, Yucatán, Mexico.
出版信息
Ecohealth. 2015 Sep;12(3):523-7. doi: 10.1007/s10393-014-1003-2. Epub 2014 Dec 24.
The aim of this study was to provide information of the occurrence of Rickettsia felis in wild mammals from three municipalities in Yucatan, Mexico. The reactivity of rodent serum to Rickettsia antigens was detected in 80.9% (17 of 21) samples using immunofluorescence assay. Polymerase chain reaction identified rickettsial DNA in spleens of 43.5% (10 of 23) rodents and 57.1% (4 of 7) opossums. The identification of the rickettsial DNA was confirmed as R. felis by restriction fragment length polymorphism and DNA sequencing. This study comprises the first report of R. felis detection in wild mammals in Yucatan.
本研究的目的是提供有关墨西哥尤卡坦半岛三个市野生哺乳动物中猫立克次体出现情况的信息。使用免疫荧光测定法在80.9%（21份样本中的17份）样本中检测到啮齿动物血清对立克次体抗原的反应性。聚合酶链反应在43.5%（23只啮齿动物中的10只）和57.1%（7只负鼠中的4只）的脾脏中鉴定出立克次体DNA。通过限制性片段长度多态性和DNA测序，立克次体DNA的鉴定被确认为猫立克次体。本研究是尤卡坦半岛野生哺乳动物中检测到猫立克次体的首次报告。
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