[微小RNA-92b对人胃癌细胞系SGC7901迁移、黏附和侵袭的影响]
[Effect of miR-92b on migration, adhesion and invasion of human gastric cancer cell line SGC7901].
作者信息
Wang Weihua, Wang Changzheng, Jiang Yi, Wu Benyan
机构信息
Department of Geriatric Gastroenterology, General Hospital of PLA, Beijing 100853, China.E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Dec;34(12):1748-52.
OBJECTIVE
To investigate the effect of miR-92b on the migration, adhesion and invasion of gastric cancer cell line SGC7901.
METHODS
The miR-92b inhibitor and mimics were transiently transfected in SGC7901 cells. The changes in the migration, adhesion and invasion of the transfected cells were tested with wound healing assay, Transwell migration assay, matrigel adhesion and Transwell invasion assay. The cellular expression of E-cadherin, vimentin, Akt and p-Akt were analyzed by Western blotting.
RESULTS
The migration, adhesion and invasion assays showed that transfection with the inhibitor of miR-92b obviously decreased the numbers of gastric cancer cells. The expression of E-cadherin, AKT, and pAKT increased and vimentin decreased significantly in the cells transfected with the inhibitor of miR-92b. Transfection with the mimics of miR-92b produced opposite effects in SGC7901 cells.
CONCLUSION
miR-92b promotes the migration, adhesion and invasion of human gastric cancer cell line SGC7901 by mediating epithelial-mesenchymal transition, and may accelerate tumor cell metastasis via signaling pathways other than PI3K/Akt pathway.
目的
探讨miR-92b对胃癌细胞系SGC7901迁移、黏附和侵袭的影响。
方法
将miR-92b抑制剂和模拟物瞬时转染至SGC7901细胞中。采用划痕愈合试验、Transwell迁移试验、基质胶黏附试验和Transwell侵袭试验检测转染细胞迁移、黏附和侵袭能力的变化。通过蛋白质免疫印迹法分析E-钙黏蛋白、波形蛋白、Akt和p-Akt的细胞表达情况。
结果
迁移、黏附和侵袭试验结果显示，转染miR-92b抑制剂后，胃癌细胞数量明显减少。转染miR-92b抑制剂的细胞中，E-钙黏蛋白、AKT和pAKT的表达增加，波形蛋白的表达显著降低。转染miR-92b模拟物后，SGC7901细胞出现相反的变化。
结论
miR-92b通过介导上皮-间质转化促进人胃癌细胞系SGC7901的迁移、黏附和侵袭，可能通过PI3K/Akt信号通路以外的其他信号通路加速肿瘤细胞转移。
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