Wen Bin, Sun Haitao, He Songqi, Cheng Yang, Jia Wenyan, Fan Eryan, Pang Jie
Department of Traditional Chinese Medicine, 458 Hospital of PLA, Guangzhou 510602, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Dec;34(12):1758-62.
To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma.
HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR.
Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities of β-catenin/TCF4 complex.
Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.
研究鳖甲煎丸对Wnt信号通路的影响及其抑制肝癌侵袭作用的机制。
用鳖甲煎丸喂养大鼠48小时后，取其血清培养HepG2细胞，采用免疫荧光法检测β-连环蛋白表达，用荧光素酶检测β-连环蛋白/TCF4复合物活性，用qRT-PCR检测下游蛋白细胞周期蛋白D1和基质金属蛋白酶-2的表达。
鳖甲煎丸处理的血清显著降低了细胞质和细胞核β-连环蛋白、细胞周期蛋白D1和基质金属蛋白酶-2蛋白的表达，并降低了β-连环蛋白/TCF4复合物的活性。
鳖甲煎丸可能是一种潜在的抗肿瘤药物，其作用可能通过抑制Wnt/β-连环蛋白通路介导。
