Zheng Guan, Liu Yang, Jing Qian, Zhang Lei
Biomedical Research Center, First People's Hospital of Kunming (Affiliated Calmette Hospital of Kunming Medical University), Yunnan, China.
Biomed Mater Eng. 2015;25(1 Suppl):145-57. doi: 10.3233/BME-141249.
The stem cell based therapy is a potential alternative to liver transplantation. The aim of the study is to investigate the hepatocytic differentiation ability of human umbilical cord derived mesenchymal stem cells (HUC-MSCs) in vitro.
The HUC-MSCs were isolated from Wharton's jelly of human umbilical cord. The cells were identified by assessing the stem cell markers. The HUC-MSCs were characterized by multipotency of differentiation. We modified the hepatogenic differentiation protocol and examine the function of differentiated cell by Periodic acid-Schiff (PAS) staining and Low-Density Lipoprotein (LDL) uptake. The protein expressions of Total protein (TP), Albumin (ALB), Globulin (GLB), Urea (BUN) and α-fetoprotein (AFP) were also detected.
The cells of the hepatogenic differentiation group showed the function of hepatocyte. Protein expressions of TP, ALB, GLB BUN and AFP improve that the HUC-MSCs are able to different into the functional hepatogenic-like cell.
The above founding indicated that the HUC-MSCs are able to different into the functional hepatogenic-like cell by present method.
基于干细胞的疗法是肝移植的一种潜在替代方法。本研究的目的是在体外研究人脐带间充质干细胞（HUC-MSCs）的肝细胞分化能力。
从人脐带的华通氏胶中分离出HUC-MSCs。通过评估干细胞标志物对细胞进行鉴定。HUC-MSCs具有多向分化潜能。我们改进了肝细胞分化方案，并通过过碘酸希夫（PAS）染色和低密度脂蛋白（LDL）摄取来检测分化细胞的功能。还检测了总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）、尿素（BUN）和甲胎蛋白（AFP）的蛋白表达。
肝细胞分化组的细胞表现出肝细胞功能。TP、ALB、GLB、BUN和AFP的蛋白表达表明HUC-MSCs能够分化为功能性肝样细胞。
上述发现表明，通过目前的方法，HUC-MSCs能够分化为功能性肝样细胞。
