Ku Lixia, Cao Liru, Wei Xiaomin, Su Huihui, Tian Zhiqiang, Guo Shulei, Zhang Liangkun, Ren Zhenzhen, Wang Xiaobo, Zhu Yuguang, Li Guohui, Wang Zhiyong, Chen Yanhui
College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China.
College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China
G3 (Bethesda). 2014 Dec 23;5(2):281-9. doi: 10.1534/g3.114.016378.
The internode length above the uppermost ear (ILAU) is an important influencing factor for canopy architecture in maize. Analyzing the genetic characteristics of internode length is critical for improving plant population structure and increasing photosynthetic efficiency. However, the genetic control of ILAU has not been determined. In this study, quantitative trait loci (QTL) for internode length at five positions above the uppermost ear were identified using four sets of recombinant inbred line (RIL) populations in three environments. Genetic maps and initial QTL were integrated using meta-analyses across the four populations. Seventy QTL were identified: 16 in population 1; 14 in population 2; 25 in population 3; and 15 in population 4. Individual effects ranged from 5.36% to 26.85% of phenotypic variation, with 27 QTL >10%. In addition, the following common QTL were identified across two populations: one common QTL for the internode length of all five positions; one common QTL for the internode length of three positions; and one common QTL for the internode length of one position. In addition, four common QTL for the internode length of four positions were identified in one population. The results indicated that the ILAU at different positions above the uppermost ear could be affected by one or several of the same QTL. The traits may also be regulated by many different QTL. Of the 70 initial QTL, 46 were integrated in 14 meta-QTL (mQTLs) by meta-analysis, and 17 of the 27 initial QTL with R(2) >10% were integrated in 7 mQTLs. Four of the key mQTLs (mQTL2-2, mQTL3-2, mQTL5-1, mQTL5-2, and mQTL9) in which the initial QTL displayed R(2) >10% included four to 11 initial QTL for an internode length of four to five positions from one or two populations. These results may provide useful information for marker-assisted selection to improve canopy architecture.
最上部雌穗以上节间长度（ILAU）是影响玉米冠层结构的重要因素。分析节间长度的遗传特性对于改善植株群体结构和提高光合效率至关重要。然而，ILAU的遗传控制尚未确定。在本研究中，利用四套重组自交系（RIL）群体，在三种环境下鉴定了最上部雌穗以上五个位置的节间长度数量性状位点（QTL）。通过对四个群体的荟萃分析，整合了遗传图谱和初始QTL。共鉴定出70个QTL：群体1中有16个；群体2中有14个；群体3中有25个；群体4中有15个。单个效应占表型变异的5.36%至26.85%，其中27个QTL>10%。此外，在两个群体中鉴定出以下共同QTL：一个是所有五个位置节间长度的共同QTL；一个是三个位置节间长度的共同QTL；一个是一个位置节间长度的共同QTL。此外，在一个群体中鉴定出四个位置节间长度的四个共同QTL。结果表明，最上部雌穗以上不同位置的ILAU可能受一个或几个相同QTL的影响。这些性状也可能受许多不同QTL的调控。在70个初始QTL中，通过荟萃分析将46个整合到14个元QTL（mQTL）中，27个R²>10%的初始QTL中有17个整合到7个mQTL中。初始QTL显示R²>10%的四个关键mQTL（mQTL2-2、mQTL3-2、mQTL5-1、mQTL5-2和mQTL9）包含来自一个或两个群体的四到五个位置节间长度的四到11个初始QTL。这些结果可能为标记辅助选择改善冠层结构提供有用信息。
