Ayad V J, Wathes D C
Department of Anatomy, Medical School, University of Bristol.
J Endocrinol. 1989 Oct;123(1):11-8. doi: 10.1677/joe.0.1230011.
Oxytocin-binding sites in the endometrium and myometrium of the non-pregnant ewe were characterized. [3H]Oxytocin bound to a single site in both tissues with high affinity; dissociation constants were determined to be 1.96 nmol/l in endometrium and 2.12 nmol/l in myometrium. Oxytocin binding was enhanced by divalent cations with a similar order of potency in both tissues: Co2+ greater than Mn2+ greater than Ni2+ greater than Mg2+ greater than Zn2+ greater than Ca2+. The endometrial and myometrial binding sites showed the same specificity for oxytocin analogues and related peptides, having high affinity for oxytocin, [Arg8]-vasopressin, [Lys8]-vasopressin, and the oxytocin-specific agonists [Gly7]-oxytocin and [Thr4,Gly7]-oxytocin. The results suggest that oxytocin receptors present in the endometrium and myometrium of the ewe are similar both to each other and to classical oxytocin receptors.
对未孕母羊子宫内膜和子宫肌层中的催产素结合位点进行了表征。[3H]催产素以高亲和力与两种组织中的单一结合位点结合；子宫内膜中的解离常数测定为1.96 nmol/l，子宫肌层中的解离常数为2.12 nmol/l。二价阳离子增强了催产素结合，两种组织中的效力顺序相似：Co2+>Mn2+>Ni2+>Mg2+>Zn2+>Ca2+。子宫内膜和子宫肌层结合位点对催产素类似物和相关肽具有相同的特异性，对催产素、[精氨酸8] - 加压素、[赖氨酸8] - 加压素以及催产素特异性激动剂[甘氨酸7] - 催产素和[苏氨酸4,甘氨酸7] - 催产素具有高亲和力。结果表明，母羊子宫内膜和子宫肌层中存在的催产素受体彼此相似，且与经典催产素受体相似。
