Carroll Chad C, Martineau Karl, Arthur Kathryn A, Huynh Richard T, Volper Brent D, Broderick Tom L
Department of Physiology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, Arizona
Department of Physiology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, Arizona.
Am J Physiol Regul Integr Comp Physiol. 2015 Feb 15;308(4):R294-9. doi: 10.1152/ajpregu.00374.2014. Epub 2014 Dec 24.
The purpose of this study was to determine whether exercise and/or acetaminophen (APAP) alter collagen and cross-linking in the rat gastrocnemius muscle, soleus muscle, and heart. Male Wistar rats (n = 50; 8 wk old) were divided into placebo (PLA) or APAP groups and sedentary (SED) or exercised (RUN) groups. APAP (200 mg/kg) was administered daily by oral gavage. Exercised groups ran on a treadmill 5 days/wk for 8 wk with progression to 60 min/day, 20 m/min, and 8° incline. Tissues were assayed for collagen (hydroxyproline) and hydroxylyslpyridinoline (HP) and lysylpyridinoline (LP) cross-links by HPLC. Collagen content (μg/mg dry weight) was greater in both the gastrocnemius (SED-PLA: 114 ± 16 vs.
RUN-PLA: 244 ± 32; P < 0.001) and soleus (SED-PLA: 51 ± 7 vs.
RUN-PLA: 99 ± 27; P = 0.005) of exercised animals. In contrast, collagen content was not significantly greater in exercised animals treated with APAP (SED-APAP: 113 ± 16 vs.
RUN-APAP: 145 ± 21) and soleus (SED-APAP: 55 ± 8 vs.
RUN-APAP: 57 ± 10). HP cross-linking (mmol/mol collagen) in the gastrocnemius (SED-PLA: 126 ± 28, RUN-PLA: 50 ± 7, SED-APAP: 41 ± 7, and
RUN-APAP: 30 ± 4) and soleus muscles (SED-PLA: 547 ± 107, RUN-PLA: 318 ± 92, SED-APAP: 247 ± 64, and
RUN-APAP: 120 ± 17) was lower in exercised rats compared with sedentary rats (P < 0.05). Cross-linking was further reduced in animals treated with APAP (P < 0.05). Neither heart collagen nor cross-linking was influenced by exercise or APAP (P > 0.05). Our findings suggest that exercise and APAP have tissue-specific effects on muscle collagen. Given the widespread use of APAP as an analgesic and antipyretic, further work in humans is warranted.
本研究的目的是确定运动和/或对乙酰氨基酚（APAP）是否会改变大鼠腓肠肌、比目鱼肌和心脏中的胶原蛋白及交联情况。雄性Wistar大鼠（n = 50；8周龄）被分为安慰剂（PLA）组或APAP组，以及久坐不动（SED）组或运动（RUN）组。通过口服灌胃每日给予APAP（200 mg/kg）。运动组大鼠每周5天在跑步机上跑步，持续8周，逐渐增加至每天60分钟、速度20米/分钟、坡度8°。通过高效液相色谱法检测组织中的胶原蛋白（羟脯氨酸）以及羟赖氨酰吡啶啉（HP）和赖氨酰吡啶啉（LP）交联情况。在腓肠肌（SED - PLA：114±16 vs. RUN - PLA：244±32；P < 0.001）和比目鱼肌（SED - PLA：51±7 vs. RUN - PLA：99±27；P = 0.005）中，运动动物的胶原蛋白含量（μg/毫克干重）更高。相比之下，接受APAP治疗的运动动物的胶原蛋白含量在腓肠肌（SED - APAP：113±16 vs. RUN - APAP：145±21）和比目鱼肌（SED - APAP：55±8 vs. RUN - APAP：57±10）中没有显著增加。与久坐不动的大鼠相比，运动大鼠腓肠肌（SED - PLA：126±28，RUN - PLA：50±7，SED - APAP：41±7，RUN - APAP：30±4）和比目鱼肌（SED - PLA：547±107，RUN - PLA：318±92，SED - APAP：247±64，RUN - APAP：120±17）中的HP交联（毫摩尔/摩尔胶原蛋白）更低（P < 0.05）。接受APAP治疗的动物交联情况进一步降低（P < 0.05）。运动或APAP对心脏胶原蛋白及交联情况均无影响（P > 0.05）。我们的研究结果表明，运动和APAP对肌肉胶原蛋白具有组织特异性影响。鉴于APAP作为镇痛药和解热药的广泛使用，有必要在人体中开展进一步研究。
