Albishri Hassan M, Abd El-Hady Deia, Tayeb Roaa A
Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 80203, Saudi Arabia
Chemistry Department, Faculty of Science-North Jeddah, King Abdulaziz University, Jeddah 80203, Saudi Arabia Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt.
J Chromatogr Sci. 2015 Aug;53(7):1123-30. doi: 10.1093/chromsci/bmu174. Epub 2014 Dec 23.
Two cyclodextrin micellar liquid chromatographic methods were developed and applied to the simultaneous determination of bisoprolol/hydrochlorothiazide and atenolol/chlorthalidone combinations in urine matrices without sample pretreatment. These combined β-blockers and diuretics chemotherapies are commonly used in the treatment of hypertension and cardiovascular diseases. Hybrid isocratic mobile phases containing hydroxypropyl-β-cyclodextrin, sodium dodecyl sulfate, phosphate buffer and methanol on a Luna C18 column with 0.5 mL min(-1) flow rate and 25.0°C column temperature were used. The methods were sensitive enough for the determination of analytes at the therapeutic urine levels with limits of detections down to 1.0 µg mL(-1); relative standard deviations and recoveries were ranged between 1.5-4.4% and 98.00-109.52%, respectively. Urinary excretion studies showed that the detection of drugs is possible up to 24 h after their ingestion. The selective proposed separations with less consumption of organic solvents over the hitherto ones could be attributed to the four point competitive interactions among analysts, pseudostationary phases and a real stationary phase.
开发了两种环糊精胶束液相色谱法，并将其应用于尿液基质中比索洛尔/氢氯噻嗪和阿替洛尔/氯噻酮组合的同时测定，无需样品预处理。这些联合的β受体阻滞剂和利尿剂化疗常用于治疗高血压和心血管疾病。在Luna C18柱上，使用含有羟丙基-β-环糊精、十二烷基硫酸钠、磷酸盐缓冲液和甲醇的混合等度流动相，流速为0.5 mL min(-1)，柱温为25.0°C。这些方法对治疗尿液水平的分析物测定足够灵敏，检测限低至1.0 µg mL(-1)；相对标准偏差和回收率分别在1.5-4.4%和98.00-109.52%之间。尿液排泄研究表明，药物摄入后24小时内都可以检测到。与以往方法相比，所提出的选择性分离方法有机溶剂消耗较少，这可能归因于分析物、假固定相和真实固定相之间的四点竞争相互作用。
