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单纯疱疹病毒1型多功能被膜蛋白UL21的异常折叠

The unusual fold of herpes simplex virus 1 UL21, a multifunctional tegument protein.

作者信息

Metrick Claire M, Chadha Pooja, Heldwein Ekaterina E

机构信息

Department of Molecular Biology and Microbiology and Graduate Program in Biochemistry, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, USA.

Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.

出版信息

J Virol. 2015 Mar;89(5):2979-84. doi: 10.1128/JVI.03516-14. Epub 2014 Dec 24.

DOI:10.1128/JVI.03516-14
PMID:25540382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4325721/
Abstract

UL21 is a conserved protein in the tegument of alphaherpesviruses and has multiple important albeit poorly understood functions in viral replication and pathogenesis. To provide a roadmap for exploration of the multiple roles of UL21, we determined the crystal structure of its conserved N-terminal domain from herpes simplex virus 1 to 2.0-Å resolution, which revealed a novel sail-like protein fold. Evolutionarily conserved surface patches highlight residues of potential importance for future targeting by mutagenesis.

摘要

UL21是α疱疹病毒被膜中的一种保守蛋白，在病毒复制和发病机制中具有多种重要但了解甚少的功能。为了提供探索UL21多种作用的路线图，我们确定了单纯疱疹病毒1 UL21保守N端结构域的晶体结构，分辨率达到2.0 Å，揭示了一种新型的帆状蛋白折叠结构。进化上保守的表面区域突出了通过诱变进行未来靶向的潜在重要残基。

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本文引用的文献

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[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.[27] 用于多同晶置换和多波长反常衍射方法的最大似然重原子参数精修
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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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Human viruses and cancer.人类病毒与癌症
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Crystal structure of the herpesvirus inner tegument protein UL37 supports its essential role in control of viral trafficking.疱疹病毒内被膜蛋白 UL37 的晶体结构支持其在控制病毒运输中的重要作用。
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The herpes simplex virus 2 UL21 protein is essential for virus propagation.单纯疱疹病毒 2 UL21 蛋白是病毒繁殖所必需的。
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The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion.单纯疱疹病毒 VP1/2 蛋白是一种动力蛋白介导的衣壳运输和神经侵袭的效应因子。
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Function of glycoprotein E of herpes simplex virus requires coordinated assembly of three tegument proteins on its cytoplasmic tail.单纯疱疹病毒糖蛋白 E 的功能需要其胞质尾部的三个被膜蛋白的协调组装。
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The C terminus of the large tegument protein pUL36 contains multiple capsid binding sites that function differently during assembly and cell entry of herpes simplex virus.巨衣壳蛋白 pUL36 的 C 端含有多个衣壳结合位点，这些位点在单纯疱疹病毒的组装和进入细胞过程中具有不同的功能。
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Dali server: conservation mapping in 3D.大理服务器：三维保护图谱构建。
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