Rasier Rifat, Gulsoy Murat
Department of Ophthalmology, Istanbul Bilim University School of Medicine, Istanbul 34349, Turkey.
Instute of Biomedical Engineering, Bogazici University, Istanbul 34684, Turkey.
Int J Ophthalmol. 2014 Dec 18;7(6):996-1000. doi: 10.3980/j.issn.2222-3959.2014.06.15. eCollection 2014.
To avoid the side effects of the suture usage by welding amniotic membrane (AM) to contact lens (CL) with laser.
AM was taken from pregnant women and cleaned from blood clots with sterile phosphate-buffered physiological saline solution which included antibiotics. Stromal side of the AM was spread inside of the CL and it was welded to CL by 1470 nm diode laser. 600 µm diameter fiber tip of the laser was contacted with the epithelial side of the AM from 4 separate points. After welding excess amniotic membrane around the CL was cut with a scalpel.
Stromal side of the AM was spread inside of the CL and then with laser fiber, different power levels and exposure times were applied on the epithelium of AM and 340 mW for seven seconds was found optimal. CL and AM attached with the spot welding effect in 4 points by touching fiber tip. CL-AM welded complex did not separated from each other while holding AM that extend beyond the CL with the help of two forceps.
As a conclusion, it was aimed in this study to achieve the success of the conventional amniotic membrane transplantation (AMT)with the easiness of applying a CL and to avoid risks and side effects of corneal or conjunctival suturing. The results showed that the application of the CL-AM complex will be as easy as the application of a CL and lasts shortly.
通过用激光将羊膜（AM）焊接到隐形眼镜（CL）上来避免缝线使用的副作用。
从孕妇身上获取羊膜，并用含抗生素的无菌磷酸盐缓冲生理盐水溶液清除血凝块。将羊膜的基质面铺展在隐形眼镜内部，并用1470纳米二极管激光将其焊接到隐形眼镜上。激光的600微米直径光纤尖端从4个不同点接触羊膜的上皮面。焊接后，用手术刀切除隐形眼镜周围多余的羊膜。
羊膜的基质面铺展在隐形眼镜内部，然后用激光光纤，对羊膜上皮施加不同功率水平和曝光时间，发现340毫瓦持续7秒为最佳。通过接触光纤尖端，隐形眼镜和羊膜在4个点产生点焊效果而附着。在两把镊子的帮助下，握住超出隐形眼镜的羊膜时，隐形眼镜 - 羊膜焊接复合体不会相互分离。
总之，本研究旨在以应用隐形眼镜的简便方式实现传统羊膜移植（AMT）的成功，并避免角膜或结膜缝合的风险和副作用。结果表明，隐形眼镜 - 羊膜复合体的应用将与应用隐形眼镜一样简便，且持续时间短。
