Drew K L, O'Connor W T, Kehr J, Ungerstedt U
Department of Pharmacology, Karolinska Institute, Stockholm, Sweden.
Life Sci. 1989;45(14):1307-17. doi: 10.1016/0024-3205(89)90134-3.
The present study characterized the voltage and calcium dependence of gamma-aminobutyric acid and dopamine overflow after the acute implantation of a microdialysis probe. Probes were implanted in dorsolateral striatum and globus pallidus. Experiments were performed under light halothane anesthesia. Basal, extracellular levels of GABA were not affected by tetrodotoxin (TTX) and were increased to 140 percent of basal values by calcium free Ringer. Basal, extracellular levels of dopamine were reduced to 14 percent of basal values by the addition of TTX and to 30 percent of basal values by the removal of calcium from the Ringer solution. The results suggest that in this in vivo preparation basal extracellular dopamine is largely of vesicular origin while GABA is not.
本研究对微透析探针急性植入后γ-氨基丁酸和多巴胺溢出的电压及钙依赖性进行了表征。将探针植入背外侧纹状体和苍白球。实验在轻度氟烷麻醉下进行。基础状态下，细胞外γ-氨基丁酸水平不受河豚毒素（TTX）影响，而无钙林格液可使其升至基础值的140%。基础状态下，加入TTX可使细胞外多巴胺水平降至基础值的14%，从林格液中去除钙则可使其降至基础值的30%。结果表明，在此体内制备中，基础细胞外多巴胺很大程度上源于囊泡，而γ-氨基丁酸并非如此。
