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[来自芽孢杆菌属微生物的位点特异性核酸内切酶的新生产者]

[New producers of site-specific endonucleases from microorganisms of the Bacillus genus].

作者信息

Kramarov V M, Skrypina N A, Smolianinov V V, Smirnov V V, Reznik S R, Sorokulova I B, Matvienko N I

出版信息

Mol Gen Mikrobiol Virusol. 1989 Jun(6):42-5.

PMID:2554130
Abstract

52 strains of Bacillus generum have been tested for production of site-specific endonucleases. The sequence recognized by the enzyme was determined for 23 enzymes, the cleavage site inside the sequence was determined for 5 enzymes. All the enzymes under study were found to be isomers of the known enzymes. The selected strains are peculiar for the high level of site-specific endonucleases content and may be used as producents of the enzymes.

摘要

已对52株芽孢杆菌属菌株进行了位点特异性核酸内切酶生产的测试。测定了23种酶所识别的序列,确定了其中5种酶在该序列内的切割位点。发现所有研究的酶都是已知酶的异构体。所选菌株的特点是位点特异性核酸内切酶含量高,可作为这些酶的生产菌株。

相似文献

1
[New producers of site-specific endonucleases from microorganisms of the Bacillus genus].[来自芽孢杆菌属微生物的位点特异性核酸内切酶的新生产者]
Mol Gen Mikrobiol Virusol. 1989 Jun(6):42-5.
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[New type II and IIs restriction endonucleases from thermophilic bacteria of the genus Bacillus].[来自芽孢杆菌属嗜热细菌的新型II型和IIs型限制性内切核酸酶]
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引用本文的文献

1
Restriction enzymes and their isoschizomers.限制性内切酶及其同裂酶。
Nucleic Acids Res. 1990 Apr 25;18 Suppl(Suppl):2331-65. doi: 10.1093/nar/18.suppl.2331.