[来自芽孢杆菌属微生物的位点特异性核酸内切酶的新生产者]
[New producers of site-specific endonucleases from microorganisms of the Bacillus genus].
作者信息
Kramarov V M, Skrypina N A, Smolianinov V V, Smirnov V V, Reznik S R, Sorokulova I B, Matvienko N I
出版信息
Mol Gen Mikrobiol Virusol. 1989 Jun(6):42-5.
PMID:2554130
Abstract
52 strains of Bacillus generum have been tested for production of site-specific endonucleases. The sequence recognized by the enzyme was determined for 23 enzymes, the cleavage site inside the sequence was determined for 5 enzymes. All the enzymes under study were found to be isomers of the known enzymes. The selected strains are peculiar for the high level of site-specific endonucleases content and may be used as producents of the enzymes.
摘要
已对52株芽孢杆菌属菌株进行了位点特异性核酸内切酶生产的测试。测定了23种酶所识别的序列，确定了其中5种酶在该序列内的切割位点。发现所有研究的酶都是已知酶的异构体。所选菌株的特点是位点特异性核酸内切酶含量高，可作为这些酶的生产菌株。
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引用本文的文献
1
Restriction enzymes and their isoschizomers.
Nucleic Acids Res. 1990 Apr 25;18 Suppl(Suppl):2331-65. doi: 10.1093/nar/18.suppl.2331.
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