骨生成素在体外对成骨和成软骨表型表达的刺激作用。
Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin.
作者信息
Vukicevic S, Luyten F P, Reddi A H
机构信息
Bone Cell Biology Section, National Institute of Dental Research, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8793-7. doi: 10.1073/pnas.86.22.8793.
Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblasts, fibroblasts, chondrocytes, and bone marrow stromal cells in vitro. Osteogenin stimulated alkaline phosphatase activity and collagen synthesis in periosteal cells. The cAMP response to parathyroid hormone in periosteal cells was increased by osteogenin. In primary cultures of calvarial osteoblasts, osteogenin stimulated alkaline phosphatase activity, the cAMP response to parathyroid hormone, and the synthesis of collagenous and noncollagenous proteins; however, cell proliferation was not affected. Osteogenin increased the production of sulfated proteoglycans in fetal rat chondroblasts and in rabbit articular chondrocytes. The present experiments demonstrate the significant influence of osteogenin in the stimulation of osteogenic and chondrogenic phenotypes in vitro.
骨生成素最近已被纯化，并测定了胰蛋白酶肽的氨基酸序列。骨生成素与不溶性胶原骨基质结合可在体内诱导软骨和骨形成。为了解骨生成素的作用机制，我们在体外研究了其对骨膜细胞、成骨细胞、成纤维细胞、软骨细胞和骨髓基质细胞的影响。骨生成素刺激骨膜细胞中的碱性磷酸酶活性和胶原蛋白合成。骨生成素增加了骨膜细胞对甲状旁腺激素的cAMP反应。在颅盖成骨细胞的原代培养中，骨生成素刺激碱性磷酸酶活性、对甲状旁腺激素的cAMP反应以及胶原蛋白和非胶原蛋白的合成；然而，细胞增殖未受影响。骨生成素增加了胎鼠软骨细胞和兔关节软骨细胞中硫酸化蛋白聚糖的产生。本实验证明了骨生成素在体外刺激成骨和软骨形成表型方面的显著影响。
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