Yuan Min, Tang Cong-Hai, Wu A-Yang, Yang Hui-Cong, Gan Wei-Wei, Zhang Tian-Xin, Huang Yan-Xue, Xu Wei-Ping
Department of Blood Transfusion, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
Department of Blood Transfusion, Southest Hospital Affiliated to Xiamen University,zhangzhou 363000, Fujian Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Dec;22(6):1716-20. doi: 10.7534/j.issn.1009-2137.2014.06.040.
In order to screen the compatible red cells by using extracorporal hemolysis test for acute autoimmune hemolytic anemia (AIHA) patients who were difficult to be matched by automatic microcolumn gel indirect antiglobulin test. Twenty-six cases of AIHA were chosen as control group, to whom the same type of donor red blood cells were infused with the weakest blood agglutination; 12 cases of acute AIHA patients were chosen as test group, these patients were difficult to be matched by automatic microcolumn gel indirect antiglobulin test, and the donor red cells without hemolysis by extracoral hemolysis test were transfused for them. The results showed that compared with the control group,the effect of transfusion was better in test group (P < 0.01), with 2.26 U leukocyte-depleted erythrocyte suspension in average, whose hemoglobin, reticulocyte and total bilirubin levels were changed significantly compared with those before blood transfusion (P < 0.01) . It is concluded that the compatible red blood cells for the acute AIHA patients can be screened by the extracorporal hemolysis test, when it is difficult to screen by the automatic microcolumn gel indirect antiglobulin test.
为了通过体外溶血试验筛选出难以用自动微柱凝胶间接抗球蛋白试验配型的急性自身免疫性溶血性贫血(AIHA)患者的相合红细胞。选取26例AIHA患者作为对照组,输注血型相同且凝集最弱的供者红细胞;选取12例急性AIHA患者作为试验组,这些患者难以用自动微柱凝胶间接抗球蛋白试验配型,为其输注经体外溶血试验无溶血的供者红细胞。结果显示,与对照组相比,试验组输血效果更好(P<0.01),平均输注去白细胞红细胞悬液2.26 U,血红蛋白、网织红细胞及总胆红素水平与输血前相比有显著变化(P<0.01)。结论是,当难以用自动微柱凝胶间接抗球蛋白试验进行筛选时,可通过体外溶血试验筛选出急性AIHA患者的相合红细胞。