Otamiri T, Tagesson C
Dept. of Occupational Medicine, Linköping University Hospital, Sweden.
Scand J Gastroenterol. 1989 Aug;24(6):666-70. doi: 10.3109/00365528909093106.
We have examined how a Ginkgo biloba extract influences the damaging effects of ischaemia in the small-intestinal mucosa. We used a rat experimental model in which a ligated loop of the distal ileum was subjected to ischaemia and revascularization, and the ensuing mucosal damage assessed by lysosomal enzyme release and intestinal permeability measurements. We also determined the mucosal content of malondialdehyde, a lipid peroxidation product, and the mucosal activity of myeloperoxidase, a neutrophil granulocyte marker. Ischaemia and revascularization alone caused increased mucosal permeability to sodium fluorescein, increased N-acetyl-beta-glucosaminidase release from the mucosa into the lumen, increased malondialdehyde content in the mucosa, and increased myeloperoxidase activity in the mucosa. Intravenous injection of G. biloba extract caused a dose-dependent attenuation of all these effects of ischaemia. It is suggested, therefore, that G. biloba extract may protect the intestinal mucosa against ischaemic damage by reducing neutrophil infiltration and lipid peroxidation.
我们研究了银杏叶提取物如何影响小肠黏膜缺血的损伤作用。我们使用了一种大鼠实验模型，其中将回肠远端的结扎肠袢进行缺血和再灌注，并通过溶酶体酶释放和肠通透性测量来评估随后的黏膜损伤。我们还测定了脂质过氧化产物丙二醛的黏膜含量以及中性粒细胞标志物髓过氧化物酶的黏膜活性。单纯的缺血和再灌注导致黏膜对荧光素钠的通透性增加、黏膜中N-乙酰-β-葡萄糖苷酶释放至肠腔增加、黏膜中丙二醛含量增加以及黏膜中髓过氧化物酶活性增加。静脉注射银杏叶提取物导致缺血的所有这些效应呈剂量依赖性减弱。因此，提示银杏叶提取物可能通过减少中性粒细胞浸润和脂质过氧化来保护肠黏膜免受缺血损伤。
