利用单克隆抗体和磁性微球对牛T淋巴细胞进行阴性富集。

Negative enrichment of bovine T lymphocytes with monoclonal antibodies and magnetic microspheres.

作者信息

Griebel P J, Gee A P, Qualtiere L, Lawman M J, Babiuk L A

机构信息

Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.

出版信息

Vet Immunol Immunopathol. 1989 Sep;22(2):161-73. doi: 10.1016/0165-2427(89)90058-5.

DOI:10.1016/0165-2427(89)90058-5

PMID:2554562

Abstract

A highly enriched population of bovine T lymphocytes was produced from peripheral blood leukocytes following the depletion of monoclonal antibody-labelled B lymphocytes and monocytes with magnetic microspheres. This negative-enrichment protocol was simple, rapid, and specific. Also, it had a high recovery efficiency and was consistently reproducible. The enriched T lymphocytes proliferated in response to recombinant bovine interleukin 2 and, following the addition of monocytes, to concanavalin A. This methodology made it possible to determine the proliferative responses of peripheral blood lymphocytes utilizing a constant number of T lymphocytes within each assay. In this way, the in vitro T lymphocyte responses were determined independent of changes in the number of responder cells within peripheral blood.

摘要

在用磁性微球去除单克隆抗体标记的B淋巴细胞和单核细胞后，从外周血白细胞中产生了高度富集的牛T淋巴细胞群体。这种阴性富集方案简单、快速且具有特异性。此外，它具有高回收效率且可始终如一地重复。富集的T淋巴细胞对重组牛白细胞介素2有增殖反应，并且在加入单核细胞后对刀豆球蛋白A有增殖反应。这种方法使得利用每次测定中恒定数量的T淋巴细胞来确定外周血淋巴细胞的增殖反应成为可能。通过这种方式，体外T淋巴细胞反应的测定不受外周血中反应细胞数量变化的影响。