Cuerda-Galindo E, Díaz-Gil G, Palomar-Gallego M A, Linares-GarcíaValdecasas R
Section of Human Anatomy and Embriology, Universidad Rey Juan Carlos, Madrid, Spain.
Section of Human Anatomy and Embriology, Universidad Rey Juan Carlos, Madrid, Spain.
Ann Anat. 2015 Mar;198:66-72. doi: 10.1016/j.aanat.2014.11.005. Epub 2014 Dec 19.
Light devices emitting near infrared have been shown to be highly effective for the skin rejuvenation but biochemical and molecular mechanism or optimum dose treatment are not well-known. In our study we try to elucidate why systems emitting near infrared produce skin improvement such as fibroblasts proliferation, increase in gene expression or extracellular matrix (ECM) protein production.
STUDY DESIGN/MATERIALS AND METHODS: 1BR3G human skin fibroblasts were used to test the effects of an intense pulsed light device emitting with an 800-1200 nm filter (MiniSilk FT manufactured by Deka(®)). In our protocol, fibroblasts were irradiated twice successively with a 10 Hz frequency, with a total fluence up to 60 J/cm(2) for 15s each pass. After incubating for 48 h, fibroblasts were harvested from the culture plates to test cell proliferation by flow cytometer. To determine changes in gene expression (mRNA levels for collagen types I and III and metalloproteinase 1 (MMP-1)) and protein production (hyaluronic acid, versican and decorin) tests were performed after irradiation.
After 48 h irradiation, 1BR3G human skin fibroblasts were observed to proliferate at a fast rate. The study of ECM macromolecules production using ELISA showed an increase of hyaluronic acid and versican production but no changes were observed for decorin. With RT-PCR assays, an increase in mRNA for collagen type I, type III and MMP-1 were observed.
Intense pulsed light emitting near infrared applied in vitro cultured cells increases fibroblasts proliferation and activity, which can be a possible mechanism of action for these devices in aging skin treatment.
已证明发射近红外光的光设备对皮肤年轻化非常有效，但生化和分子机制或最佳剂量治疗尚不清楚。在我们的研究中，我们试图阐明为什么发射近红外光的系统会产生皮肤改善，如成纤维细胞增殖、基因表达增加或细胞外基质（ECM）蛋白产生增加。
研究设计/材料与方法：使用1BR3G人皮肤成纤维细胞来测试发射800 - 1200nm滤光片的强脉冲光设备（由Deka(®)制造的MiniSilk FT）的效果。在我们的实验方案中，成纤维细胞以10Hz频率连续照射两次，每次照射15秒，总能量密度高达60J/cm²。孵育48小时后，从培养板中收获成纤维细胞，通过流式细胞仪测试细胞增殖。为了确定基因表达的变化（I型和III型胶原蛋白以及金属蛋白酶1（MMP - 1）的mRNA水平）和蛋白质产生（透明质酸、多功能蛋白聚糖和核心蛋白聚糖），在照射后进行测试。
照射48小时后，观察到1BR3G人皮肤成纤维细胞快速增殖。使用酶联免疫吸附测定法对ECM大分子产生的研究表明透明质酸和多功能蛋白聚糖的产生增加，但未观察到核心蛋白聚糖的变化。通过逆转录 - 聚合酶链反应测定，观察到I型、III型胶原蛋白和MMP - 1的mRNA增加。
体外培养细胞中应用发射近红外光的强脉冲光可增加成纤维细胞的增殖和活性，这可能是这些设备在治疗衰老皮肤中的一种作用机制。
